Abstract：
This study aimed to encapsulate Talaromyces amestolkiae colorants in maltodextrin and chitosan microparticles using the spraydrying technique and to evaluate the biopolymers\' capacities to protect the fungal colorant against temperature (65 °C) and extreme pH (2.0 and 13.0). The compact microparticles exhibited smooth or indented surfaces with internal diameters ranging between 2.58-4.69 μm and ζ ~ -26 mV. The encapsulation efficiencies were 86 % and 56 % for chitosan and maltodextrin microparticles, respectively. The shifted endothermic peaks of the free colorants indicated their physical stabilization into microparticles. The encapsulated colorants retained most of their absorbance (compared to the 0 h) even after 25 days at 65 °C. Contrary, the free colorant presented almost no absorbance after 1 day under the same conditions. Colorants in chitosan and maltodextrin matrices also partially maintained their colorimetric and fluorometric properties at acidic pH. However, only maltodextrin improved the resistance of the red colorant to alkaline environments. For the first time, the potential of polysaccharide-based microparticles to preserve polyketide colorants was demonstrated using 3D fluorescence. Therefore, this study demonstrated an alternative in developing functional products with natural color additives.
摘要：
本研究旨在使用喷雾干燥技术将amestolkiae着色剂封装在麦芽糖糊精和壳聚糖微粒中，并评估生物聚合物保护真菌着色剂免受温度（65°C）和极端pH（2.0和13.0）的能力。致密的微粒表现出光滑或锯齿状的表面，内径在2.58-4.69μm和ζ〜26mV之间。壳聚糖和麦芽糖糊精微粒的包封效率分别为86%和56%，分别。游离着色剂的移动吸热峰表明它们在微粒中的物理稳定性。即使在65°C下25天后，包封的着色剂仍保留了大部分的吸光度（与0小时相比）。相反，在相同条件下1天后,游离着色剂几乎没有吸光度。壳聚糖和麦芽糖糊精基质中的着色剂在酸性pH下也部分保持其比色和荧光性质。然而,只有麦芽糊精提高了红色着色剂对碱性环境的抗性。第一次,使用3D荧光证明了基于多糖的微粒保存聚酮化合物着色剂的潜力。因此,这项研究证明了开发具有天然色素添加剂的功能性产品的替代方案。
