Abstract：
A method involving chitosan-assisted magnetic-stirring-enhanced mechanical amorphous dispersion extraction was developed and utilized to extract hydrophobic anthraquinones from Rhei Radix et Rhizoma prior to ultrahigh performance liquid chromatography analysis. Incorporating natural chitosan as a dispersant facilitated the extraction of hydrophobic anthraquinones using purified water, considerably enhancing the eco-friendliness of the extraction methodology. To optimize extraction efficiency, an extensive evaluation of the crucial parameters influencing rhubarb yield was conducted. Furthermore, a response surface methodology was applied to optimize the extraction conditions. Under these optimized conditions, the method exhibited linearity ranges of 0.1-100 µg/mL, with correlation coefficients between 0.9990 and 0.9998. The method\'s intraday (n = 6) and interday (n = 6) precision levels were maintained at ≤3.58%, which was considered to be within acceptable limits. The computed detection and quantification limits were 16.54-24.60 and 54.91-82.04 ng/mL, respectively. Consequently, this optimized method was effectively employed to extract five specific compounds (aloe-emodin, emodin, rhein, chrysophanol, and physcion) from Rhei Radix et Rhizoma, achieving recoveries ranging from 86.43% to 102.75%.
摘要：
开发了一种涉及壳聚糖辅助磁力搅拌增强机械无定形分散体提取的方法，并在超高效液相色谱分析之前将其用于提取大黄中的疏水性蒽醌。掺入天然壳聚糖作为分散剂有助于使用纯净水提取疏水性蒽醌，大大提高了提取方法的生态友好性。优化提取效率,对影响大黄产量的关键参数进行了广泛的评估。此外，采用响应面法优化提取条件。在这些优化条件下，该方法的线性范围为0.1-100µg/mL，相关系数在0.9990和0.9998之间。该方法的日内(n=6)和日内(n=6)精度保持在≤3.58%，这被认为是在可接受的范围内。计算的检测和定量限分别为16.54-24.60和54.91-82.04ng/mL，分别。因此,这种优化的方法被有效地用于提取五种特定的化合物(芦荟大黄素，大黄素,rhein,大黄酚,和physcion）来自大黄，回收率从86.43%到102.75%不等。
