Abstract：
In order to explore the in vivo anti-food allergy activity of Lactobacillus sakei subsp. sakei-fermented Eucheuma spinosum polysaccharides F1-ESP-3, an ovalbumin (OVA)-induced food allergy mouse model was established by ascites immunization and gavage. The weight, temperature, incidence of diarrhea, levels of allergic mediators and inflammatory factors in the serum of mice were analyzed. We analyzed the differentiation of mouse spleen lymphocytes and the proportion of sensitized mast cells by flow cytometry. The intestinal barrier status of mice was analyzed by intestinal pathological tissue sections and microbiota sequencing. The results showed that F1-ESP-3 could alleviate the food allergy symptoms of mice, such as hypothermia and loose stool; levels of OVA-specific immunoglobulin E, mast cell protease and histamine in the serum of sensitized mice and the proportion of dendritic cells and mast cells in mouse spleen were significantly reduced; in addition, F1-ESP-3 may protect the intestinal barrier and further improve the intestinal microenvironment of food-allergic mice by regulating the abundance of Bacteroidetes and Firmicutes. F1-ESP-3 can further improve the intestinal microenvironment of food-allergic mice by upregulating the levels of Lachnospiraceae, and may affect the signal pathways such as NOD-like receptor, MAPK, I kappa B and antigen processing and presentation.
摘要：
为了探讨沙棘乳杆菌亚种的体内抗食物过敏活性。通过腹水免疫和灌胃，建立了一种卵清蛋白（OVA）诱导的食物过敏小鼠模型。重量,温度,腹泻的发病率，分析小鼠血清中过敏介质和炎症因子的水平。我们通过流式细胞术分析了小鼠脾淋巴细胞的分化和致敏肥大细胞的比例。通过肠道病理组织切片和微生物区系测序分析小鼠肠道屏障状态。结果表明,F1-ESP-3能缓解小鼠食物过敏症状,如低温和稀便；OVA特异性免疫球蛋白E的水平，致敏小鼠血清中的肥大细胞蛋白酶和组胺以及小鼠脾脏中树突状细胞和肥大细胞的比例显著降低;F1-ESP-3可能通过调节拟杆菌和厚壁菌的丰度，保护肠道屏障，进一步改善食物过敏小鼠的肠道微环境。F1-ESP-3可通过上调Lachnospiraceae的水平进一步改善食物过敏小鼠的肠道微环境，并可能影响信号通路，如NOD样受体，MAPK,IκB与抗原加工和呈递。
