Abstract：
We describe four cases of a novel carbapenem-resistant Pseudomonas aeruginosa ST179 clone carrying the blaKPC-2 or blaKPC-35 gene together with blaIMP-16, imported from Peru to Spain and isolated from leukemia patients. All isolates were multidrug-resistant but remained susceptible to fosfomycin, cefiderocol, and colistin. Whole-genome sequencing revealed that blaKPC-2 and blaKPC-35 were located in an IncP6 plasmid, whereas blaIMP-16 was in a chromosomal type 1 integron. This study highlights the global threat of multidrug-resistant P. aeruginosa clones and underscores the importance of monitoring and early detection of emerging resistance mechanisms to guide appropriate treatment strategies. The importation and spread of such clones emphasize the urgent need to implement strict infection control measures to prevent the dissemination of carbapenem-resistant bacteria.
OBJECTIVE: This is the first documented case of a Pseudomonas aeruginosa ST179 strain carrying the blaKPC-35 gene, and it represents the first report of a P. aeruginosa co-harboring blaIMP-16 and either blaKPC-2 or blaKPC-35, which wre imported from Peru to Spain, highlighting a threat due to the capacity of spreading carbapenem-resistance via plasmid conjugation.
OBJECTIVE: This is the first documented case of a Pseudomonas aeruginosa ST179 strain carrying the blaKPC-35 gene, and it represents the first report of a P. aeruginosa co-harboring blaIMP-16 and either blaKPC-2 or blaKPC-35, which wre imported from Peru to Spain, highlighting a threat due to the capacity of spreading carbapenem-resistance via plasmid conjugation.
摘要：
我们描述了四例新型耐碳青霉烯的铜绿假单胞菌ST179克隆，该克隆携带blaKPC-2或blaKPC-35基因以及blaIMP-16，从秘鲁进口到西班牙，并从白血病患者中分离出来。所有分离株都是多重耐药的，但仍然对磷霉素敏感，cefiderocol,还有粘菌素.全基因组测序显示blaKPC-2和blaKPC-35位于IncP6质粒中，而blaIMP-16位于染色体1型整合子中。这项研究强调了多重耐药铜绿假单胞菌克隆的全球威胁，并强调了监测和早期发现新兴耐药机制以指导适当治疗策略的重要性。此类克隆的输入和传播强调迫切需要实施严格的感染控制措施，以防止碳青霉烯类耐药细菌的传播。
目的：这是第一例携带blaKPC-35基因的铜绿假单胞菌ST179菌株，它代表了从秘鲁进口到西班牙的铜绿假单胞菌共同藏有blaIMP-16和blaKPC-2或blaKPC-35的第一份报告，突出了通过质粒接合传播碳青霉烯抗性的能力所带来的威胁。
目的：这是第一例携带blaKPC-35基因的铜绿假单胞菌ST179菌株，它代表了从秘鲁进口到西班牙的铜绿假单胞菌共同藏有blaIMP-16和blaKPC-2或blaKPC-35的第一份报告，突出了通过质粒接合传播碳青霉烯抗性的能力所带来的威胁。
