Abstract：
OBJECTIVE: Collagen has long been recognized as an excellent carrier for growth factors, and membrane-type collagen has been widely applied in dentistry for guided bone regeneration. This study was conducted to examine the effects of an activin A/BMP2 chimera (AB204) combined with a collagen membrane (CM) on bone repair in a rat calvarial defect model.
METHODS: A unilateral calvarial defect measuring 5.0 mm was surgically created in 32 Sprague-Dawley rats. The rats were then randomly assigned to 1 of 4 groups, each consisting of 8 animals: control (untreated), CM (treated with a CM only), CM/bone morphogenetic protein 2 (BMP2) (treated with a CM and 1.0 μg of BMP2), and CM/AB204 (treated with a CM and 1.0 μg of AB204). Bone regeneration was evaluated using micro-computed tomography (CT) and histological analysis at 2 and 4 weeks following surgery.
RESULTS: Micro-CT analysis revealed that bone formation in the CM/BMP2 and CM/AB204 groups was superior to that observed in the control and CM groups at both 2 and 4 weeks postoperatively. BMP2 induced greater bone regeneration than AB204 at 2 weeks; however, AB204 resulted in a greater bone volume at 4 weeks, achieving the highest values recorded. No significant differences were found between the CM/BMP2 and CM/AB204 groups at either time point (P>0.05). On histological examination, new bone formation was evident in both CM/BMP2 and CM/AB204 groups.
CONCLUSIONS: Within the limitations of this study, the findings indicate that AB204 may enhance osteogenic potential when used in combination with CM for bone regeneration.
METHODS: A unilateral calvarial defect measuring 5.0 mm was surgically created in 32 Sprague-Dawley rats. The rats were then randomly assigned to 1 of 4 groups, each consisting of 8 animals: control (untreated), CM (treated with a CM only), CM/bone morphogenetic protein 2 (BMP2) (treated with a CM and 1.0 μg of BMP2), and CM/AB204 (treated with a CM and 1.0 μg of AB204). Bone regeneration was evaluated using micro-computed tomography (CT) and histological analysis at 2 and 4 weeks following surgery.
RESULTS: Micro-CT analysis revealed that bone formation in the CM/BMP2 and CM/AB204 groups was superior to that observed in the control and CM groups at both 2 and 4 weeks postoperatively. BMP2 induced greater bone regeneration than AB204 at 2 weeks; however, AB204 resulted in a greater bone volume at 4 weeks, achieving the highest values recorded. No significant differences were found between the CM/BMP2 and CM/AB204 groups at either time point (P>0.05). On histological examination, new bone formation was evident in both CM/BMP2 and CM/AB204 groups.
CONCLUSIONS: Within the limitations of this study, the findings indicate that AB204 may enhance osteogenic potential when used in combination with CM for bone regeneration.
摘要：
目的：胶原蛋白长期以来被认为是生长因子的优良载体，膜型胶原在牙科引导骨再生中得到了广泛的应用。进行这项研究是为了检查激活素A/BMP2嵌合体（AB204）与胶原膜（CM）结合对大鼠颅骨缺损模型中骨修复的影响。
方法：在32只Sprague-Dawley大鼠中通过手术产生测量为5.0mm的单侧颅骨缺损。然后将大鼠随机分配到4组中的1组,每个由8只动物组成：对照（未治疗），CM（仅使用CM处理），CM/骨形态发生蛋白2（BMP2）（用CM和1.0μgBMP2处理），和CM/AB204(用CM和1.0μgAB204处理)。在手术后2周和4周使用显微计算机断层扫描（CT）和组织学分析评估骨再生。
结果：Micro-CT分析显示，在术后2周和4周，CM/BMP2和CM/AB204组的骨形成优于对照组和CM组。BMP2在2周时比AB204诱导更大的骨再生;然而，AB204在4周时导致更大的骨体积，达到记录的最高值。CM/BMP2组和CM/AB204组各时间点差异均无统计学意义(P>0.05)。在组织学检查中，CM/BMP2和CM/AB204组均有明显的新骨形成。
结论：在本研究的局限性内，研究结果表明,AB204与CM联合用于骨再生时可增强成骨潜能.
方法：在32只Sprague-Dawley大鼠中通过手术产生测量为5.0mm的单侧颅骨缺损。然后将大鼠随机分配到4组中的1组,每个由8只动物组成：对照（未治疗），CM（仅使用CM处理），CM/骨形态发生蛋白2（BMP2）（用CM和1.0μgBMP2处理），和CM/AB204(用CM和1.0μgAB204处理)。在手术后2周和4周使用显微计算机断层扫描（CT）和组织学分析评估骨再生。
结果：Micro-CT分析显示，在术后2周和4周，CM/BMP2和CM/AB204组的骨形成优于对照组和CM组。BMP2在2周时比AB204诱导更大的骨再生;然而，AB204在4周时导致更大的骨体积，达到记录的最高值。CM/BMP2组和CM/AB204组各时间点差异均无统计学意义(P>0.05)。在组织学检查中，CM/BMP2和CM/AB204组均有明显的新骨形成。
结论：在本研究的局限性内，研究结果表明,AB204与CM联合用于骨再生时可增强成骨潜能.
