PDF(Pubmed)
Abstract：
Tissue engineering includes the construction of tissue-organ scaffold. The advantage of three-dimensional scaffolds over two-dimensional scaffolds is that they provide homeostasis for a longer time. The microbial community in Symbiotic culture of bacteria and yeast (SCOBY) can be a source for kombucha (kombu tea) production. In this study, it was aimed to investigate the usage of SCOBY, which produces bacterial cellulose, as a biomaterial and 3D scaffold material. 3D printable biomaterial was obtained by partial hydrolysis of oolong tea and black tea kombucha biofilms. In order to investigate the usage of 3D kombucha biomaterial as a tissue scaffold, \"L929 cell line 3D cell culture\" was created and cell viability was tested in the biomaterial. At the end of the 21st day, black tea showed 51% and oolong tea 73% viability. The cytotoxicity of the materials prepared by lyophilizing oolong and black tea kombucha beverages in fibroblast cell culture was determined. Black tea IC50 value: 7.53 mg, oolong tea IC50 value is found as 6.05 mg. Fibroblast viability in 3D biomaterial + lyophilized oolong and black tea kombucha beverages, which were created using the amounts determined to these values, were investigated by cell culture Fibroblasts in lyophilized and 3D biomaterial showed viability of 58% in black tea and 78% in oolong tea at the end of the 7th day. In SEM analysis, it was concluded that fibroblast cells created adhesion to the biomaterial. 3D biomaterial from kombucha mushroom culture can be used as tissue scaffold and biomaterial.
摘要：
组织工程包括组织-器官支架的构建。三维支架优于二维支架的优点是它们提供了更长的时间内的稳态。细菌和酵母的共生培养物（SCOBY）中的微生物群落可以成为康普茶（康布茶）生产的来源。在这项研究中,它的目的是调查SCOBY的用法，产生细菌纤维素，作为生物材料和3D支架材料。通过乌龙茶和红茶红茶菌生物膜的部分水解获得3D打印生物材料。为了研究3D红茶菌生物材料作为组织支架的用途，创建了“L929细胞系3D细胞培养”，并在生物材料中测试了细胞活力。在第21天结束的时候,红茶和乌龙茶的存活率分别为51%和73%。测定了冻干乌龙茶和红茶红茶茶饮料制备的材料在成纤维细胞培养中的细胞毒性。红茶IC50值：7.53毫克，乌龙茶IC50值为6.05mg。3D生物材料+冻干乌龙茶和红茶茶饮料中的成纤维细胞活力，使用这些值确定的数量创建的，通过细胞培养研究冻干和3D生物材料中的成纤维细胞在第7天结束时在红茶中显示58％和乌龙茶中78％的活力。在SEM分析中，结论是成纤维细胞与生物材料产生粘附。红茶菌培养的3D生物材料可用作组织支架和生物材料。
