PDF(Pubmed)
Abstract：
Antimicrobial coatings provide protection against microbes colonization on surfaces. This can prevent the stabilization and proliferation of microorganisms. The ever-increasing levels of microbial resistance to antimicrobials are urging the development of alternative types of compounds that are potent across broad spectra of microorganisms and target different pathways. This will help to slow down the development of resistance and ideally halt it. The development of composite antimicrobial coatings (CACs) that can host and protect various antimicrobial agents and release them on demand is an approach to address this urgent need. In this work, new CACs based on microsized hybrids of calcium carbonate (CaCO3) and silver nanoparticles (AgNPs) were designed using a drop-casting technique. Polyvinylpyrrolidone and mucin were used as additives. The CaCO3/AgNPs hybrids contributed to endowing colloidal stability to the AgNPs and controlling their release, thereby ensuring the antibacterial activity of the coatings. Moreover, the additives PVP and mucin served as a matrix to (i) control the distribution of the hybrids, (ii) ensure mechanical integrity, and (iii) prevent the undesired release of AgNPs. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) techniques were used to characterize the 15 μm thick CAC. The antibacterial activity was determined against Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa, three bacteria responsible for many healthcare infections. Antibacterial performance of the hybrids was demonstrated at concentrations between 15 and 30 μg/cm2. Unloaded CaCO3 also presented bactericidal properties against MRSA. In vitro cytotoxicity tests demonstrated that the hybrids at bactericidal concentrations did not affect human dermal fibroblasts and human mesenchymal stem cell viability. In conclusion, this work presents a simple approach for the design and testing of advanced multicomponent and functional antimicrobial coatings that can protect active agents and release them on demand.
摘要：
抗微生物涂层提供防止微生物在表面上定殖的保护。这可以防止微生物的稳定和增殖。不断增加的微生物对抗菌剂的抗性水平正在促使开发替代类型的化合物，这些化合物在广谱的微生物中都有效，并靶向不同的途径。这将有助于减缓阻力的发展，并理想地停止阻力。开发能够承载和保护各种抗微生物剂并按需释放它们的复合抗微生物涂料(CAC)是解决这一迫切需要的方法。在这项工作中,使用滴注技术设计了基于碳酸钙（CaCO3）和银纳米颗粒（AgNPs）的微型杂化物的新型CAC。聚乙烯吡咯烷酮和粘蛋白用作添加剂。CaCO3/AgNP杂种有助于赋予AgNP胶体稳定性并控制其释放，从而保证涂料的抗菌活性。此外，添加剂PVP和粘蛋白作为基质，以（i）控制杂种的分布，(ii)确保机械完整性，和(iii)防止AgNP的不希望的释放。扫描电子显微镜（SEM），X射线衍射(XRD)和傅里叶变换红外（FTIR）技术用于表征15μm厚的CAC。对大肠杆菌的抗菌活性进行了测定，耐甲氧西林金黄色葡萄球菌(MRSA),铜绿假单胞菌,导致许多医疗保健感染的三种细菌。在15至30μg/cm2的浓度下证明了杂种的抗菌性能。未负载的CaCO3还呈现针对MRSA的杀菌性质。体外细胞毒性测试表明，杀菌浓度的杂种不会影响人真皮成纤维细胞和人间充质干细胞的活力。总之,这项工作提出了一种简单的方法，用于设计和测试先进的多组分和功能性抗菌涂料，可以保护活性剂，并释放他们的需求。
