PDF(Pubmed)
Abstract：
Carotenoids play essential roles in plant growth and development and provide plants with a tolerance to a series of abiotic stresses. In this study, the function and biological significance of lycopene β-cyclase, lycopene ε-cyclase, and β-carotene hydroxylase, which are responsible for the modification of the tetraterpene skeleton procedure, were isolated from Lycium chinense and analyzed. The overexpression of lycopene β-cyclase, lycopene ε-cyclase, and β-carotene hydroxylase promoted the accumulation of total carotenoids and photosynthesis enhancement, reactive oxygen species scavenging activity, and proline content of tobacco seedlings after exposure to the salt stress. Furthermore, the expression of the carotenoid biosynthesis genes and stress-related genes (ascorbate peroxidase, catalase, peroxidase, superoxide dismutase, and pyrroline-5-carboxylate reductase) were detected and showed increased gene expression level, which were strongly associated with the carotenoid content and reactive oxygen species scavenging activity. After exposure to salt stress, the endogenous abscisic acid content was significantly increased and much higher than those in control plants. This research contributes to the development of new breeding aimed at obtaining stronger salt tolerance plants with increased total carotenoids and vitamin A content.
摘要：
类胡萝卜素在植物的生长发育中起着至关重要的作用，并为植物提供对一系列非生物胁迫的耐受性。在这项研究中,番茄红素β-环化酶的功能和生物学意义,番茄红素ε-环化酶，和β-胡萝卜素羟化酶，它们负责四萜烯骨架程序的修改，从枸杞中分离和分析。番茄红素β-环化酶的过表达,番茄红素ε-环化酶，β-胡萝卜素羟化酶促进总类胡萝卜素的积累和光合作用的增强,活性氧清除活性，和暴露于盐胁迫后烟草幼苗的脯氨酸含量。此外，类胡萝卜素生物合成基因和胁迫相关基因的表达(抗坏血酸过氧化物酶，过氧化氢酶,过氧化物酶,超氧化物歧化酶,和吡咯啉-5-羧酸还原酶）被检测到，并显示出增加的基因表达水平，与类胡萝卜素含量和活性氧清除活性密切相关。暴露于盐胁迫后，内源脱落酸含量显着增加，并且远高于对照植物。这项研究有助于开发新的育种，旨在获得具有增加的总类胡萝卜素和维生素A含量的更耐盐植物。
