PDF(Pubmed)
Abstract：
Sinking or floating is the natural state of planktonic organisms and particles in the ocean. Simulating these conditions is critical when making measurements, such as respirometry, because they allow the natural exchange of substrates and products between sinking particles and water flowing around them and prevent organisms that are accustomed to motion from changing their metabolism. We developed a rotating incubator, the RotoBOD (named after its capability to rotate and determine biological oxygen demand, BOD), that uniquely enables automated oxygen measurements in small volumes while keeping the samples in their natural state of suspension. This allows highly sensitive rate measurements of oxygen utilization and subsequent characterization of single particles or small planktonic organisms, such as copepods, jellyfish, or protists. As this approach is nondestructive, it can be combined with several further measurements during and after the incubation, such as stable isotope additions and molecular analyses. This makes the instrument useful for ecologists, biogeochemists, and potentially other user groups such as aquaculture facilities. Here, we present the technical background of our newly developed apparatus and provide examples of how it can be utilized to determine oxygen production and consumption in small organisms and particles.
摘要：
下沉或漂浮是海洋中浮游生物和颗粒的自然状态。进行测量时，模拟这些条件至关重要，比如呼吸测量法，因为它们允许在下沉的颗粒和周围流动的水之间自然交换底物和产物，并防止习惯于运动的生物体改变其新陈代谢。我们开发了一个旋转培养箱，Rotobod(以其旋转和确定生物需氧量的能力命名，BOD),这独特地实现了小体积的自动氧气测量，同时保持样品的自然悬浮状态。这样可以对氧气利用率进行高灵敏度的测量，并随后对单个颗粒或小型浮游生物进行表征，比如co足类动物，水母,或者原生生物。由于这种方法是非破坏性的，它可以与孵育期间和之后的几个进一步的测量相结合，如稳定同位素添加和分子分析。这使得仪器对生态学家有用，生物地球化学家,以及可能的其他用户群体，如水产养殖设施。这里,我们介绍了我们新开发的仪器的技术背景，并提供了如何利用它来确定小生物和颗粒中氧气的产生和消耗的例子。
