PDF(Pubmed)
Abstract：
Corticoids have found their way into the globe of sports, due to their anti-inflammatory properties, and have often found to be added to dietary supplements for illegally improving the effectiveness of their products. Earlier studies describe the detection of corticoids in several matrices, but this can be an incessant and continuous process as long because the doping practices continue. In this study, we report a technique to verify concurrently 44 of the foremost commonly abused synthetic corticoids (including chiral analogs) in equine plasma supported chiral liquid chromatography-electrospray ionization mass spectrometry. Polysaccharide i-cellulose-5 column was used for chromatographic separation with a gradient mode. The validation studies were also meted out by using equine plasma so as to judge the suitability of the strategy. Detection limits were determined between 0.01 and 0.05 ng/mL and therefore the limit of quantification was between 0.1 and 0.5 ng/mL. Recovery and matrix effect on the analytes was further assessed. Since the developed method was ready to separate the corticoids and to differentiate chiral analogs at very low levels (in picograms), this separation techniques may be employed for the determination (confirmatory analysis) of the corticoids in the forensic and anti-doping application.
摘要：
皮质已经进入了体育运动的全球，由于它们的抗炎特性，并且经常被发现添加到膳食补充剂中以非法提高其产品的有效性。早期的研究描述了几种基质中皮质激素的检测，但这可能是一个不断和连续的过程，因为兴奋剂的做法继续。在这项研究中,我们报道了一项技术,在马血浆支持的手性液相色谱-电喷雾电离质谱中同时验证44种最常见的滥用合成皮质激素(包括手性类似物).多糖i-纤维素-5柱用于梯度模式的色谱分离。还通过使用马血浆进行了验证研究，以判断该策略的适用性。测定的检出限为0.01-0.05ng/mL,因此定量限为0.1-0.5ng/mL。进一步评估了对分析物的回收率和基质效应。由于所开发的方法已准备好分离皮质激素并以非常低的水平（皮克）区分手性类似物，这种分离技术可用于法医和反兴奋剂应用中皮质激素的测定(确证分析)。
