PDF(Pubmed)
Abstract：
BACKGROUND: Mosquito-borne viruses cause various infectious diseases in humans and animals. Oya virus (OYAV) and Ebinur Lake virus (EBIV), belonging to the genus Orthobunyavirus within the family Peribunyaviridae, are recognized as neglected viruses with the potential to pose threats to animal or public health. The evaluation of vector competence is essential for predicting the arbovirus transmission risk.
METHODS: To investigate the range of mosquito vectors for OYAV (strain SZC50) and EBIV (strain Cu20-XJ), the susceptibility of four mosquito species (Culex pipiens pallens, Cx. quinquefasciatus, Aedes albopictus, and Ae. aegypti) was measured through artificial oral infection. Then, mosquito species with a high infection rate (IR) were chosen to further evaluate the dissemination rate (DR), transmission rate (TR), and transmission efficiency. The viral RNA in each mosquito sample was determined by RT-qPCR.
RESULTS: The results revealed that for OYAV, Cx. pipiens pallens had the highest IR (up to 40.0%) among the four species, but the DR and TR were 4.8% and 0.0%, respectively. For EBIV, Cx. pipiens pallens and Cx. quinquefasciatus had higher IR compared to Ae. albopictus (1.7%). However, the EBIV RNA and infectious virus were detected in Cx. pipiens pallens, with a TR of up to 15.4% and a transmission efficiency of 3.3%.
CONCLUSIONS: The findings indicate that Cx. pipiens pallens was susceptible to OYAV but had an extremely low risk of transmitting the virus. Culex pipiens pallens and Cx. quinquefasciatus were susceptible to EBIV, and Cx. pipiens pallens had a higher transmission risk to EBIV than Cx. quinquefasciatus.
METHODS: To investigate the range of mosquito vectors for OYAV (strain SZC50) and EBIV (strain Cu20-XJ), the susceptibility of four mosquito species (Culex pipiens pallens, Cx. quinquefasciatus, Aedes albopictus, and Ae. aegypti) was measured through artificial oral infection. Then, mosquito species with a high infection rate (IR) were chosen to further evaluate the dissemination rate (DR), transmission rate (TR), and transmission efficiency. The viral RNA in each mosquito sample was determined by RT-qPCR.
RESULTS: The results revealed that for OYAV, Cx. pipiens pallens had the highest IR (up to 40.0%) among the four species, but the DR and TR were 4.8% and 0.0%, respectively. For EBIV, Cx. pipiens pallens and Cx. quinquefasciatus had higher IR compared to Ae. albopictus (1.7%). However, the EBIV RNA and infectious virus were detected in Cx. pipiens pallens, with a TR of up to 15.4% and a transmission efficiency of 3.3%.
CONCLUSIONS: The findings indicate that Cx. pipiens pallens was susceptible to OYAV but had an extremely low risk of transmitting the virus. Culex pipiens pallens and Cx. quinquefasciatus were susceptible to EBIV, and Cx. pipiens pallens had a higher transmission risk to EBIV than Cx. quinquefasciatus.
摘要：
背景：蚊媒病毒在人和动物中引起各种传染病。奥雅病毒（OYAV）和艾比湖病毒（EBIV），属于Peribunyaviridae家族中的直鼻病毒属，被认为是被忽视的病毒，有可能对动物或公共健康构成威胁。媒介能力的评估对于预测虫媒病毒传播风险至关重要。
方法：为了调查OYAV（SZC50株）和EBIV（Cu20-XJ株）的蚊媒范围，四种蚊子的易感性(淡色库蚊，Cx.Quinquefasciatus,白纹伊蚊,还有Ae.埃及伊蚊）是通过人工口腔感染进行测量的。然后，选择具有高感染率（IR）的蚊子物种来进一步评估传播率（DR），传输速率(TR)，和传输效率。通过RT-qPCR测定每个蚊子样品中的病毒RNA。
结果：结果显示，对于OYAV，Cx.pipienspallens在这四个物种中具有最高的IR（高达40.0％），但DR和TR分别为4.8%和0.0%，分别。对于EBIV，Cx.pipiens苍白和Cx。quinquefasciatus的IR高于Ae。白纹伊蚊(1.7%)。然而,在Cx中检测到EBIVRNA和感染性病毒。pipienspallens,TR高达15.4%，传输效率为3.3%。
结论：研究结果表明，CX。pipienspallens对OYAV易感，但传播该病毒的风险极低。淡色库蚊和Cx。quinquefasciatus易感EBIV，Cx.pipienspallens比Cx具有更高的EBIV传播风险。Quinquefasciatus.
方法：为了调查OYAV（SZC50株）和EBIV（Cu20-XJ株）的蚊媒范围，四种蚊子的易感性(淡色库蚊，Cx.Quinquefasciatus,白纹伊蚊,还有Ae.埃及伊蚊）是通过人工口腔感染进行测量的。然后，选择具有高感染率（IR）的蚊子物种来进一步评估传播率（DR），传输速率(TR)，和传输效率。通过RT-qPCR测定每个蚊子样品中的病毒RNA。
结果：结果显示，对于OYAV，Cx.pipienspallens在这四个物种中具有最高的IR（高达40.0％），但DR和TR分别为4.8%和0.0%，分别。对于EBIV，Cx.pipiens苍白和Cx。quinquefasciatus的IR高于Ae。白纹伊蚊(1.7%)。然而,在Cx中检测到EBIVRNA和感染性病毒。pipienspallens,TR高达15.4%，传输效率为3.3%。
结论：研究结果表明，CX。pipienspallens对OYAV易感，但传播该病毒的风险极低。淡色库蚊和Cx。quinquefasciatus易感EBIV，Cx.pipienspallens比Cx具有更高的EBIV传播风险。Quinquefasciatus.
