PDF(Pubmed)
Abstract：
BACKGROUND: Sturgeon species are living fossils that exhibit unique reproductive characteristics, and elucidation of the molecular processes governing the formation and quality of sturgeon eggs is crucial. However, comprehensive data on the protein composition of sturgeon ovarian fluid (OF) and eggs and their functional significance are lacking. To address this knowledge gap, the aim of the present study was to conduct a comprehensive comparative proteomic analysis of Siberian sturgeon OF and eggs using liquid chromatography-mass spectrometry (LC-MS/MS).
RESULTS: A total of 617 proteins were identified in OF, and 565 proteins were identified in eggs. A total of 772 proteins showed differential abundance. Among the differentially abundant proteins, 365 were more abundant in OFs, while 407 were more abundant in eggs. We identified 339 proteins unique to OFs and 287 proteins specific to eggs, and further investigated the top 10 most abundant proteins in each. The functional annotation of the OF proteins highlighted their predominant association with immune system processes, including the complement and coagulation cascade, neutrophil and leukocyte-mediated immunity, cholesterol metabolism, and regulation of the actin cytoskeleton. Analysis of egg proteins revealed enrichment in metabolic pathways, such as oxidative phosphorylation and fatty acid metabolism, and protein ubiquitination and translation. OF-specific proteins included extracellular matrix and secretory vesicles, and eggs were enriched in proteins localized to mitochondria and ribosome components.
CONCLUSIONS: This study presents the first comprehensive characterization of the protein composition of sturgeon OF and eggs and elucidates their distinct functional roles. These findings advance our understanding of sturgeon reproduction, OF-egg signaling and the origin of OF proteins. The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier PXD044168 to ensure accessibility for further research.
RESULTS: A total of 617 proteins were identified in OF, and 565 proteins were identified in eggs. A total of 772 proteins showed differential abundance. Among the differentially abundant proteins, 365 were more abundant in OFs, while 407 were more abundant in eggs. We identified 339 proteins unique to OFs and 287 proteins specific to eggs, and further investigated the top 10 most abundant proteins in each. The functional annotation of the OF proteins highlighted their predominant association with immune system processes, including the complement and coagulation cascade, neutrophil and leukocyte-mediated immunity, cholesterol metabolism, and regulation of the actin cytoskeleton. Analysis of egg proteins revealed enrichment in metabolic pathways, such as oxidative phosphorylation and fatty acid metabolism, and protein ubiquitination and translation. OF-specific proteins included extracellular matrix and secretory vesicles, and eggs were enriched in proteins localized to mitochondria and ribosome components.
CONCLUSIONS: This study presents the first comprehensive characterization of the protein composition of sturgeon OF and eggs and elucidates their distinct functional roles. These findings advance our understanding of sturgeon reproduction, OF-egg signaling and the origin of OF proteins. The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier PXD044168 to ensure accessibility for further research.
摘要：
背景：St鱼物种是具有独特生殖特征的活化石，阐明控制st鱼卵形成和质量的分子过程至关重要。然而,缺乏有关st鱼卵巢液（OF）和卵的蛋白质组成及其功能意义的全面数据。为了解决这个知识差距，本研究的目的是使用液相色谱-质谱(LC-MS/MS)对西伯利亚st和鸡蛋进行全面的比较蛋白质组学分析.
结果：在OF中总共鉴定出617种蛋白质，在鸡蛋中鉴定出565种蛋白质。总共772种蛋白质显示出不同的丰度。在差异丰富的蛋白质中，365在OF中更丰富，而407个鸡蛋更丰富。我们鉴定了339种OFs特有的蛋白质和287种卵特有的蛋白质，并进一步研究了每种蛋白质中最丰富的前10种蛋白质。OF蛋白的功能注释强调了它们与免疫系统过程的主要关联，包括补体和凝血级联，中性粒细胞和白细胞介导的免疫，胆固醇代谢，和肌动蛋白细胞骨架的调节。卵蛋白的分析揭示了代谢途径的富集，如氧化磷酸化和脂肪酸代谢，以及蛋白质泛素化和翻译。OF特异性蛋白包括细胞外基质和分泌囊泡，卵富含定位于线粒体和核糖体成分的蛋白质。
结论：这项研究首次全面描述了st鱼和卵的蛋白质组成，并阐明了它们不同的功能作用。这些发现促进了我们对st鱼繁殖的理解，OF-卵信号和OF蛋白的起源。质谱蛋白质组学数据已与数据集标识符PXD044168一起存放在ProteomeXchange联盟中，以确保进一步研究的可访问性。
结果：在OF中总共鉴定出617种蛋白质，在鸡蛋中鉴定出565种蛋白质。总共772种蛋白质显示出不同的丰度。在差异丰富的蛋白质中，365在OF中更丰富，而407个鸡蛋更丰富。我们鉴定了339种OFs特有的蛋白质和287种卵特有的蛋白质，并进一步研究了每种蛋白质中最丰富的前10种蛋白质。OF蛋白的功能注释强调了它们与免疫系统过程的主要关联，包括补体和凝血级联，中性粒细胞和白细胞介导的免疫，胆固醇代谢，和肌动蛋白细胞骨架的调节。卵蛋白的分析揭示了代谢途径的富集，如氧化磷酸化和脂肪酸代谢，以及蛋白质泛素化和翻译。OF特异性蛋白包括细胞外基质和分泌囊泡，卵富含定位于线粒体和核糖体成分的蛋白质。
结论：这项研究首次全面描述了st鱼和卵的蛋白质组成，并阐明了它们不同的功能作用。这些发现促进了我们对st鱼繁殖的理解，OF-卵信号和OF蛋白的起源。质谱蛋白质组学数据已与数据集标识符PXD044168一起存放在ProteomeXchange联盟中，以确保进一步研究的可访问性。
