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Abstract：
Unlike coding genes, the number of lncRNA genes in organism genomes is relatively proportional to organism complexity. From plants to humans, the tissues with highest numbers and levels of lncRNA gene expression are the male reproductive organs. To learn why, we initiated a genome-wide analysis of Drosophila lncRNA spatial expression patterns in these tissues. The numbers of genes and levels of expression observed greatly exceed those previously reported, due largely to a preponderance of non-polyadenylated transcripts. In stark contrast to coding genes, the highest numbers of lncRNAs expressed are in post-meiotic spermatids. Correlations between expression levels, localization and previously performed genetic analyses indicate high levels of function and requirement. More focused analyses indicate that lncRNAs play major roles in evolution by controlling transposable element activities, Y chromosome gene expression and sperm construction. A new type of lncRNA-based particle found in seminal fluid may also contribute to reproductive outcomes.
摘要：
与编码基因不同，生物体基因组中lncRNA基因的数量与生物体的复杂性成正比。从植物到人类,lncRNA基因表达数量和水平最高的组织是男性生殖器官。要了解为什么，我们启动了果蝇lncRNA在这些组织中的空间表达模式的全基因组分析。观察到的基因数量和表达水平大大超过了以前的报道，主要是由于非聚腺苷酸化转录物的优势。与编码基因形成鲜明对比的是，在减数分裂后的精子细胞中表达的lncRNAs数量最高。表达水平之间的相关性，定位和以前进行的遗传分析表明，高水平的功能和要求。更集中的分析表明，lncRNAs通过控制转座元件活性在进化中发挥主要作用，Y染色体基因表达与精子构建。在精液中发现的一种新型的基于lncRNA的颗粒也可能有助于生殖结果。
