Abstract：
Micro(nano)plastic, as a new type of environmental pollutant, have become a potential threat to the life and health of various stages of biology. However, it is not yet clear whether they will affect brain development in the fetal stage. Therefore, this study aims to explore the potential effects of nanoplastics on the development of fetal rat brains. To assess the allocation of NPs (25 nm and 50 nm) in various regions of the fetal brain, pregnant rats were exposed to concentrations (50, 10, 2.5, and 0.5 mg/kg) of PS-NPs. Our results provided evidence of the transplacental transfer of PS-NPs to the fetal brain, with a prominent presence observed in several cerebral regions, notably the cerebellum, hippocampus, striatum, and prefrontal cortex. This distribution bias might be linked to the developmental sequence of each brain region. Additionally, we explored the influence of prenatal exposure on the myelin development of the cerebellum, given its the highest PS-NP accumulation in offspring. Compared with control rats, PS-NPs exposure caused a significant reduction in myelin basic protein (MBP) and myelin oligodendrocyte glycoprotein (MOG) expression, a decrease in myelin thickness, an increase in cell apoptosis, and a decline in the oligodendrocyte population. These effects gave rise to motor deficits. In conclusion, our results identified the specific distribution of NPs in the fetal brain following prenatal exposure and revealed that prenatal exposure to PS-NPs can suppress myelin formation in the cerebellum of the fetus.
摘要：
微（纳米）塑料，作为一种新型的环境污染物，已成为生物学各个阶段的生命和健康的潜在威胁。然而,目前尚不清楚它们是否会影响胎儿期的大脑发育。因此,本研究旨在探讨纳米塑料对胎鼠脑发育的潜在影响。为了评估胎儿大脑各个区域的NP（25nm和50nm）的分配，怀孕大鼠暴露于浓度(50,10,2.5,和0.5mg/kg)的PS-NP。我们的结果提供了PS-NP经胎盘转移到胎儿大脑的证据，在几个大脑区域观察到突出的存在，尤其是小脑，海马体,纹状体,和前额叶皮层.这种分布偏差可能与每个大脑区域的发育顺序有关。此外,我们探讨了产前暴露对小脑髓鞘发育的影响，鉴于其后代中PS-NP积累量最高。与对照组大鼠相比,PS-NP暴露导致髓鞘碱性蛋白（MBP）和髓鞘少突胶质细胞糖蛋白（MOG）表达显着降低，髓鞘厚度减少，细胞凋亡的增加，少突胶质细胞数量的减少。这些影响导致了运动缺陷。总之,我们的结果确定了产前暴露后NPs在胎儿脑中的特定分布,并表明产前暴露于PS-NPs可以抑制胎儿小脑的髓鞘形成.
