PDF(Pubmed)
Abstract：
Tobacco use significantly influences the oral microbiome. However, less is known about how different tobacco products specifically impact the oral microbiome over time. To address this knowledge gap, we characterized the oral microbiome of cigarette users, smokeless tobacco users, and non-users over 4 months (four time points). Buccal swab and saliva samples (n = 611) were collected from 85 participants. DNA was extracted from all samples and sequencing was carried out on an Illumina MiSeq, targeting the V3-V4 region of the 16S rRNA gene. Cigarette and smokeless tobacco users had more diverse oral bacterial communities, including a higher relative abundance of Firmicutes and a lower relative abundance of Proteobacteria, when compared to non-users. Non-users had a higher relative abundance of Actinomyces, Granulicatella, Haemophilus, Neisseria, Oribacterium, Prevotella, Pseudomonas, Rothia, and Veillonella in buccal swab samples, compared to tobacco users. While the most abundant bacterial genera were relatively constant over time, some species demonstrated significant shifts in relative abundance between the first and last time points. In addition, some opportunistic pathogens were detected among tobacco users including Neisseria subflava, Bulleidia moorei and Porphyromonas endodontalis. Overall, our results provide a more holistic understanding of the structure of oral bacterial communities in tobacco users compared to non-users.
摘要：
烟草使用显著影响口腔微生物组。然而,人们对不同的烟草产品如何随着时间的推移具体影响口腔微生物组知之甚少。为了解决这个知识差距，我们对香烟使用者的口腔微生物组进行了表征，无烟烟草使用者,和非用户超过4个月（四个时间点）。收集85名参与者的口腔拭子和唾液样本（n=611）。从所有样品中提取DNA，并在IlluminaMiSeq上进行测序，靶向16SrRNA基因的V3-V4区域。香烟和无烟烟草使用者的口腔细菌群落更加多样化，包括较高的Firmicutes相对丰度和较低的变形杆菌相对丰度，与非用户相比。非使用者放线菌的相对丰度较高，肉芽肿,嗜血杆菌,奈瑟菌,杆菌,普雷沃氏菌,假单胞菌,Rothia,和颊拭子样本中的Veillonella,与烟草使用者相比。虽然最丰富的细菌属随着时间的推移相对恒定，一些物种在第一个和最后一个时间点之间表现出相对丰度的显著变化。此外,在烟草使用者中检测到一些机会性病原体，包括亚黄奈瑟菌，球藻和根管卟啉菌。总的来说,我们的研究结果提供了对烟草使用者和非烟草使用者口腔细菌群落结构更全面的了解.
