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Abstract：
Introduction Clinically, the early prediction of the severity of COVID-19 is often challenging, as a dramatic change in severity can occur without warning. The severity of COVID-19 disease is associated with an increased level of inflammatory mediators, including cytokines. This study aimed to evaluate the association of the levels of cytokines interleukin (IL)-2, IL-6, tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), and IL-10 with the severity of COVID-19 in Bangladesh. Materials and methods This cross-sectional study included a total of 60 confirmed cases of COVID-19, comprising 30 severe cases (Group A) and 30 non-severe cases (Group B), and 10 healthy individuals (Group C) attending Bangabandhu Sheikh Mujib Medical University (BSMMU) from March 2021 to February 2022. The cytokine assay was performed using the human Th1/Th2/Th17 cytokine kit BD cytometric bead array (CBA) on the BD Accuri C6 Plus flow cytometer. Statistical analysis was conducted using IBM SPSS Statistics for Windows, Version 23 (Released 2015; IBM Corp., Armonk, New York). Results The mean ages of the patients in Groups A, B, and C were 60.73±5.97, 57.13±7.68, and 48.10±9.13 years, respectively, with a male predominance in all groups. The mean IL-2, IL-6, IL-10, and TNF-α levels had a positive correlation with the increased age group and male gender, although it was not statistically significant. The mean IL-6 and IFN-γ levels were significantly higher among severe cases (216.95±147.78 and 0.98±0.95 pg/mL, respectively) compared to non-severe cases (94.29±128.79 and 0.41±0.61 pg/mL, respectively) and healthy individuals (1.08±1.97 and 0.15±0.28 pg/mL, respectively). Furthermore, the anti-inflammatory cytokine IL-10 was also significantly higher among severe cases (17.92±21.87 pg/mL) compared to non-severe cases (5.38±6.73 pg/mL) and healthy individuals (1.62±1.65 pg/mL). Conclusion IL-6, IFN-γ, and IL-10 have a significant association with the severity of COVID-19 disease. Clinicians treating patients with COVID-19 can consider the level of these cytokines as biomarkers of severity.
摘要：
临床介绍,对COVID-19严重程度的早期预测通常具有挑战性，严重程度的戏剧性变化可能会在没有警告的情况下发生。COVID-19疾病的严重程度与炎症介质水平升高有关，包括细胞因子。本研究旨在评估细胞因子白细胞介素（IL）-2，IL-6，肿瘤坏死因子-α（TNF-α）水平的相关性。干扰素-γ（IFN-γ），和IL-10与孟加拉国COVID-19的严重程度有关。材料与方法本横断面研究共纳入60例确诊COVID-19病例，包括30例重症病例（A组）和30例非重症病例（B组），以及从2021年3月至2022年2月参加BangabandhuSheikhMujib医科大学（BSMMU）的10名健康个体（C组）。使用人Th1/Th2/Th17细胞因子试剂盒BD细胞计数珠阵列(CBA)在BDAccuriC6Plus流式细胞仪上进行细胞因子测定。使用IBMSPSSStatisticsforWindows进行统计分析，版本23（2015年发布；IBMCorp.,Armonk,纽约）。结果A组患者的平均年龄,B,C分别为60.73±5.97,57.13±7.68,48.10±9.13年,分别,在所有群体中都以男性为主。平均IL-2、IL-6、IL-10和TNF-α水平与增龄组、男性性别呈正相关,虽然没有统计学意义。在重症病例中，平均IL-6和IFN-γ水平显着升高（216.95±147.78和0.98±0.95pg/mL，分别）与非重症病例（94.29±128.79和0.41±0.61pg/mL，分别）和健康个体（1.08±1.97和0.15±0.28pg/mL，分别)。此外，与非重症病例(5.38±6.73pg/mL)和健康个体(1.62±1.65pg/mL)相比,重症病例(17.92±21.87pg/mL)的抗炎细胞因子IL-10也显著更高.结论IL-6、IFN-γ、和IL-10与COVID-19疾病的严重程度有显著关联。治疗COVID-19患者的临床医生可以将这些细胞因子的水平视为严重程度的生物标志物。
