UNASSIGNED: Adult male Sprague-Dawley rats were randomly assigned to a single mTBI impact, RSC impact, sham, or repeated sham groups.
UNASSIGNED: Following a weight drop injury, anxiety-like behavior and general locomotive activity and were assessed using the open field test, while motor coordination was evaluated using a rotarod unit. Neuronal loss, astrogliosis, and microgliosis were assessed using NeuN, GFAP and Iba-1 immunohistochemistry. All assessments were undertaken at 3- and 7-days post impact.
UNASSIGNED: No behavioral disturbances were observed in injury groups, however, both injury groups did lead to microgliosis following 3-days post-impact.
UNASSIGNED: No pathophysiological differences were observed between a single mTBI impact and RSC impacts of the same energy input. Even though a cumulative injury threshold for RSC impacts was not determined, a threshold still may exist where no pathodynamic shift occurs.
■成年雄性Sprague-Dawley大鼠被随机分配到单个mTBI冲击中,RSC影响,sham,或重复的假团体。
■体重下降后受伤,焦虑样行为和一般机车活动,并使用开场测试进行评估,而运动协调是使用旋转杆单元进行评估的。神经元丢失,星形胶质增生,和小胶质细胞增生使用NeuN进行评估,GFAP和Iba-1免疫组织化学。所有评估均在影响后3天和7天进行。
■在损伤组中未观察到行为障碍,然而,两个损伤组均在撞击后3天后导致小胶质细胞增生.
■在相同能量输入的单个mTBI冲击和RSC冲击之间没有观察到病理生理学差异。即使RSC影响的累积损伤阈值尚未确定,阈值仍然可能存在,其中没有发生病理动态偏移。