{Reference Type}: Journal Article
{Title}: Short-term behavioral and histological findings following a single concussive and repeated subconcussive brain injury in a rodent model.
{Author}: Clay AM;Carr RL;DuBien JL;To F;
{Journal}: Brain Inj
{Volume}: 38
{Issue}: 10
{Year}: 2024 Aug 23
{Factor}: 2.167
{DOI}: 10.1080/02699052.2024.2349144
{Abstract}: <B>UNASSIGNED: </B>It is unclear of the correlation between a mild traumatic brain injury (mTBI) and repeated subconcussive (RSC) impacts with respect to injury biomechanics. Thus, the present study was designed to determine the behavioral and histological differences between a single mTBI impact and RSC impacts with subdivided cumulative kinetic energies of the single mTBI impact.<BR><B>UNASSIGNED: </B>Adult male Sprague-Dawley rats were randomly assigned to a single mTBI impact, RSC impact, sham, or repeated sham groups.<BR><B>UNASSIGNED: </B>Following a weight drop injury, anxiety-like behavior and general locomotive activity and were assessed using the open field test, while motor coordination was evaluated using a rotarod unit. Neuronal loss, astrogliosis, and microgliosis were assessed using NeuN, GFAP and Iba-1 immunohistochemistry. All assessments were undertaken at 3- and 7-days post impact.<BR><B>UNASSIGNED: </B>No behavioral disturbances were observed in injury groups, however, both injury groups did lead to microgliosis following 3-days post-impact.<BR><B>UNASSIGNED: </B>No pathophysiological differences were observed between a single mTBI impact and RSC impacts of the same energy input. Even though a cumulative injury threshold for RSC impacts was not determined, a threshold still may exist where no pathodynamic shift occurs.