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Abstract：
Biosurfactants, as microbial bioproducts, have significant potential in the field of microbial enhanced oil recovery (MEOR). Biosurfactants are microbial bioproducts with the potential to reduce the interfacial tension (IFT) between crude oil and water, thus enhancing oil recovery. This study aims to investigate the production and characterization of biosurfactants and evaluate their effectiveness in increasing oil recovery. Pseudoxanthomonas taiwanensis was cultured on SMSS medium to produce biosurfactants. Crude oil was found to be the most effective carbon source for biosurfactant production. The biosurfactants exhibited comparable activity to sodium dodecyl sulfate (SDS) at a concentration of 400 ppm in reducing IFT. It was characterized as glycolipids, showing stability in emulsions at high temperatures (up to 120 °C), pH levels ranging from 3 to 9, and NaCl concentrations up to 10% (w/v). Response surface methodology revealed the optimized conditions for the most stable biosurfactants (pH 7, temperature of 40 °C, and salinity of 2%), resulting in an EI24 value of 64.45%. Experimental evaluations included sand pack column and core flooding studies, which demonstrated additional oil recovery of 36.04% and 12.92%, respectively. These results indicate the potential application of P. taiwanensis biosurfactants as sustainable and environmentally friendly approaches to enhance oil recovery in MEOR processes.
摘要：
生物表面活性剂,作为微生物生物产品，在微生物强化采油(MEOR)领域具有巨大的潜力。生物表面活性剂是微生物生物产品，具有降低原油和水之间的界面张力（IFT）的潜力，从而提高石油采收率。本研究旨在研究生物表面活性剂的生产和表征，并评估其在提高石油采收率方面的有效性。在SMSS培养基上培养黄原假单胞菌以生产生物表面活性剂。发现原油是生产生物表面活性剂最有效的碳源。生物表面活性剂在400ppm的浓度下在降低IFT方面表现出与十二烷基硫酸钠(SDS)相当的活性。它的特征是糖脂，在高温（高达120°C）的乳液中显示出稳定性，pH值范围为3至9，NaCl浓度高达10％（w/v）。响应面法揭示了最稳定的生物表面活性剂的优化条件（pH7，温度40°C，盐度为2%)，导致EI24值为64.45%。实验评估包括砂充填柱和岩心驱油研究，这表明额外的石油采收率为36.04%和12.92%，分别。这些结果表明，台湾假单胞菌生物表面活性剂作为可持续和环境友好的方法来提高MEOR工艺中的石油采收率的潜在应用。
