PDF(Pubmed)
Abstract：
Presenting new molecular and scanning electron microscope (SEM) features, this study gives additional data to the better knowledge of Thaparocleidus vistulensis (Siwak, 1932) (Monopisthocotyla, Ancylodiscoididae), a parasite of the European catfish Silurus glanis Linnaeus, 1758 (Siluriformes, Siluridae) cultured in a commercial fish farm in Hungary. In addition, notes on the early development of sclerotized anchors are also provided. The main morphological difference of T. vistulensis compared to other congeneric species is associated with the male copulatory organ, which exhibits 5-7 loops in the middle of the penis length and a long open V-shaped sclerotized accessory piece, dividing terminally into two parts, securing the terminal part of the penis tube. The present study provides for the first time molecular characterization data based on the 2694 bp long nucleotide sequence of rDNA (ITS1, 5.8S, ITS2, and flanked with partial 18S and partial 28S) submitted in GenBank with the accession number OR916383. A phylogenetic tree based on ITS1 sequences supports a well-defined clade including T. vistulensis, forming a sister group with T. siluri, a species-specific monopisthocotylan parasite to S. glanis. The morphological characterization of T. vistulensis, especially for the male copulatory organ, together with the molecular data in the present study, extends knowledge about this monopisthocotylan species and provides new information for future phylogeny studies.
摘要：
提出了新的分子和扫描电子显微镜（SEM）特征，这项研究提供了更多的数据，以更好地了解Thaparocledusvistulensis(Siwak，1932年）（单端球藻，盘状球菌），欧洲鲶鱼SilurusglanisLinnaeus的寄生虫，1758年（Siluriformes，Siluridae）在匈牙利的商业养鱼场中养殖。此外,还提供了关于硬化锚早期发展的说明。与其他同属物种相比，悬臂梁的主要形态差异与雄性交配器官有关，在阴茎长度的中间有5-7个环和一个长的开放的V形硬化配件，最终分为两部分，固定阴茎管的末端部分。本研究首次提供了基于2694bp长的rDNA核苷酸序列的分子表征数据(ITS1，5.8S，ITS2，侧翼为部分18S和部分28S），在GenBank中提交，登录号为OR916383。基于ITS1序列的系统发育树支持明确定义的进化枝，包括T.vistulensis，和T.siluri组成姐妹团,S.glanis的一种特定物种的单性逆叶多糖寄生虫。悬臂梁的形态特征，尤其是男性交配器官,连同本研究中的分子数据，扩展了有关这种单向型cootylan物种的知识，并为未来的系统发育研究提供了新的信息。
