PDF(Pubmed)
Abstract：
Coccidiosis, a protozoan disease that substantially impacts poultry production, is characterized by an intracellular parasite. The study utilized 48 one-day-old Horro chickens, randomly divided into the infected (I) and control (C) groups. The challenge group of chickens were administered Eimeria maxima oocysts via oral gavage at 21-days-old, and each chicken received 2 mL containing 7×104 sporulated oocysts. The total RNAs of chicken jejunum and cecum tissues were isolated from three samples, each from I and C groups. Our study aimed to understand the host immune-parasite interactions and compare immune response mRNA profiles in chicken jejunum and cecum tissues at 4 and 7 days postinfection with Eimeria maxima. The results showed that 823 up- and 737 down-regulated differentially expressed mRNAs (DEmRNAs) in jejunum at 4 d infection and control (J4I vs. J4C), and 710 up- and 368 down-regulated DEmRNAs in jejunum at 7 days infection and control (J7I vs. J7C) were identified. In addition, DEmRNAs in cecum tissue, 1424 up- and 1930 down-regulated genes in cecum at 4 days infection and control (C4I vs. C4C), and 77 up- and 191 down-regulated genes in cecum at 7 days infection and control (C7I vs. C7C) were detected. The crucial DEmRNAs, including SLC7A5, IL1R2, GLDC, ITGB6, ADAMTS4, IL1RAP, TNFRSF11B, IMPG2, WNT9A, and FOXF1, played pivotal roles in the immune response during Eimeria maxima infection of chicken jejunum. In addition, the potential detection of FSTL3, RBP7, CCL20, DPP4, PRKG2, TFPI2, and CDKN1A in the cecum during the host immune response against Eimeria maxima infection is particularly noteworthy. Furthermore, our functional enrichment analysis revealed the primary involvement of DEmRNAs in small molecule metabolic process, immune response function, inflammatory response, and toll-like receptor 10 signaling pathway in the jejunum at 4 and 7 days postinfection. Similarly, in the cecum, DEmRNAs at 4 and 7 days postinfection were enriched in processes related to oxidative stress response and immune responses. Our findings provide new insights and contribute significantly to the field of poultry production and parasitology.
摘要：
球虫病,一种严重影响家禽生产的原生动物疾病，以细胞内寄生虫为特征。这项研究使用了48只一天大的Horro鸡，随机分为感染组(I)和对照组(C)。攻击组鸡在21日龄时通过口服管饲法给予最大艾美球虫卵囊，每只鸡接受2毫升含有7×104个孢子形成的卵囊。从三个样本中分离鸡空肠和盲肠组织的总RNA,各I组和C组。我们的研究旨在了解宿主免疫与寄生虫的相互作用，并比较鸡空肠和盲肠组织在感染艾美球虫后4天和7天的免疫反应mRNA谱。结果表明，在感染和对照4d时，空肠中823个上调和737个下调差异表达的mRNA（DEmRNAs）（J4Ivs.J4C),在7天感染和对照时，空肠中710个上调的DEmRNAs和368个下调的DEmRNAs(J7Ivs.J7C）进行了鉴定。此外,盲肠组织中的DMRNAs，感染和对照4天时盲肠中1424个上调基因和1930个下调基因（C4Ivs.C4C),在7天感染和对照时，盲肠中77个上调基因和191个下调基因(C7Ivs.检测到C7C)。关键的DMRNAs,包括SLC7A5、IL1R2、GLDC、ITGB6,ADAMTS4,IL1RAP,TNFRSF11B,IMPG2,WNT9A,和FOXF1在鸡空肠最大艾美球虫感染期间的免疫应答中起关键作用。此外,在针对最大艾美球虫感染的宿主免疫应答过程中,盲肠中FSTL3,RBP7,CCL20,DPP4,PRKG2,TFPI2和CDKN1A的潜在检测尤其值得注意.此外，我们的功能富集分析揭示了DMRNAs主要参与小分子代谢过程，免疫反应功能，炎症反应，感染后4天和7天空肠中的Toll样受体10信号通路。同样，在盲肠，在感染后4天和7天,在与氧化应激反应和免疫反应相关的过程中富集DMnRNA。我们的发现提供了新的见解，并为家禽生产和寄生虫学领域做出了重大贡献。
