Abstract：
Production of carotenoids by yeast fermentation is an advantaged technology due to its easy scaling and safety. Nevertheless, carotenoid production needs an economic culture medium and other efficient yeast stains. The study aims to isolate and identify a yeast strain capable of producing carotenoids using a cost-effective substrate. A new strain was identified as Rhodotorula toruloides L/24-26-1, which can produce carotenoids at different pretreated and unpretreated sugarcane molasses concentrations (40 and 80 g/L). The highest biomass concentration (18.6 ± 0.6 g/L) was reached in the culture using 80 g/L of hydrolyzed molasses. On the other hand, the carotenoid accumulation reached the maximum value using pretreated molasses at 40 g/L (715.4 ± 15.1 µg/g d.w). In this case, the β-carotene was 1.5 times higher than that on the control medium. The yeast growth in molasses was not correlated with carotenoid production. The most outstanding production of The DPPH, ABTS, and FRAP tests demonstrated the antioxidant activity of the obtained carotenogenic extracts. This research demonstrated the R. toruloides L/24-26-1 strain biotechnological potential for carotenoid compounds. The yeast produces carotenoids with antioxidant activity in an inexpensive medium, such as sulfuric acid pretreated and unpretreated molasses.
摘要：
通过酵母发酵生产类胡萝卜素由于其易于结垢和安全性而成为一种有利的技术。然而，类胡萝卜素的生产需要经济的培养基和其他有效的酵母染色剂。该研究旨在分离和鉴定能够使用具有成本效益的底物生产类胡萝卜素的酵母菌株。鉴定出一种新菌株为RhodotorulatoruloidesL/24-26-1，该菌株可以在不同的预处理和未预处理的甘蔗糖蜜浓度（40和80g/L）下产生类胡萝卜素。使用80g/L的水解糖蜜在培养物中达到最高生物量浓度(18.6±0.6g/L)。另一方面,使用40g/L（715.4±15.1µg/gd.w）的预处理糖蜜，类胡萝卜素积累达到最大值。在这种情况下,β-胡萝卜素比对照培养基高1.5倍。糖蜜中的酵母生长与类胡萝卜素的产生无关。最杰出的DPPH生产，ABTS,和FRAP测试证明了所获得的类胡萝卜素提取物的抗氧化活性。这项研究证明了R.toluloidesL/24-26-1菌株对类胡萝卜素化合物的生物技术潜力。酵母在廉价的培养基中产生具有抗氧化活性的类胡萝卜素，如硫酸预处理和未预处理的糖蜜。
