Abstract：
In the wake of rapid industrialization and burgeoning transportation networks, the escalating demand for fossil fuels has accelerated the depletion of finite energy reservoirs, necessitating urgent exploration of sustainable alternatives. To address this, current research is focusing on renewable fuels like second-generation bioethanol from agricultural waste such as sugarcane bagasse. This approach not only circumvents the contentious issue of food-fuel conflicts associated with biofuels but also tackles agricultural waste management. In the present study indigenous yeast strain, Clavispora lusitaniae QG1 (MN592676), was isolated from rotten grapes to ferment xylose sugars present in the hemicellulose content of sugarcane bagasse. To liberate the xylose sugars, dilute acid pretreatment was performed. The highest reducing sugars yield was 1.2% obtained at a temperature of 121 °C for 15 min, a solid-to-liquid ratio of 1:25 (% w/v), and an acid concentration of 1% dilute acid H2SO4 that was significantly higher (P < 0.001) yield obtained under similar conditions at 100 °C for 1 h. The isolated strain was statistically optimized for fermentation process by Plackett-Burman design to achieve the highest ethanol yield. Liberated xylose sugars were completely utilized by Clavispora lusitaniae QG1 (MN592676) and gave 100% ethanol yield. This study optimizes both fermentation process and pretreatment of sugarcane bagasse to maximize bioethanol yield and demonstrates the ability of isolated strain to effectively utilize xylose as a carbon source. The desirable characteristics depicted by strain Clavispora lusitaniae shows its promising utilization in management of industrial waste like sugarcane bagasse by its conversion into renewable biofuels like bioethanol.
摘要：
随着快速工业化和交通网络的蓬勃发展，对化石燃料的不断增长的需求加速了有限能源的枯竭，迫切需要探索可持续的替代方案。为了解决这个问题，目前的研究重点是可再生燃料，如从甘蔗渣等农业废物中提取的第二代生物乙醇。这种方法不仅避免了与生物燃料相关的食品燃料冲突的争议问题，而且还解决了农业废物管理问题。在本研究中，土著酵母菌株，lusitaniaeQG1（MN592676），从腐烂的葡萄中分离出来，以发酵甘蔗渣半纤维素含量中存在的木糖糖。为了释放木糖糖，进行稀酸预处理。在121°C的温度下持续15分钟，最高的还原糖产率为1.2％，固液比为1:25(%w/v)，和酸浓度为1％的稀酸H2SO4，在类似条件下在100°C下1h获得的产量显着提高（P<0.001）。通过Plackett-Burman设计对分离的菌株进行了统计学优化，用于发酵过程，以实现最高的乙醇产量。分离的木糖糖完全由ClavisporalusitanieQG1（MN592676）利用，并得到100％的乙醇产率。这项研究优化了甘蔗渣的发酵过程和预处理，以最大程度地提高生物乙醇产量，并证明了分离菌株有效利用木糖作为碳源的能力。Clavisporalusitaniae菌株所描绘的理想特征表明，通过将其转化为生物乙醇等可再生生物燃料，可将其用于处理甘蔗渣等工业废物。
