关键词： cystic fibrosis gene expression omnibus infection nontuberculous mycobacteria transcription factors

Mesh ： Cystic Fibrosis / microbiology complications Humans Mycobacterium Infections, Nontuberculous / microbiology Nontuberculous Mycobacteria / genetics Protein Interaction Maps Receptors, CXCR4 / genetics metabolism Gene Expression Profiling Transcription Factors / genetics metabolism

来  源：   DOI：10.2217/fmb-2023-0237   PDF(Pubmed)

Abstract：
Aim: This study aims to explore the molecular mechanisms of cystic fibrosis (CF) complicated with nontuberculous mycobacteria (NTM) infection. Materials & methods: Expression profiles of CF with NTM-infected patients were downloaded from GEO database. Intersection analysis yielded 78 genes associated with CF with NTM infection. The protein-protein interaction (PPI) network and the functions of hub genes were investigated. Results: Five hub genes (PIK3R1, IL1A, CXCR4, ACTN1, PFN1) were identified, which were primarily enriched in actin-related biological processes and pathways. Transcription factors RELA, JUN, NFKB1 and FOS that regulated hub genes modulated IL1A expression, while 21 other transcription factors regulated CXCR4 expression. Conclusion: In summary, this study may provide new insights into the mechanisms of CF with NTM infection.
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摘要：
目的:探讨囊性纤维化(CF)合并非结核分枝杆菌(NTM)感染的分子机制。材料和方法:从GEO数据库下载具有NTM感染的患者的CF的表达谱。交叉分析产生了与具有NTM感染的CF相关的78个基因。研究了蛋白质-蛋白质相互作用（PPI）网络和hub基因的功能。结果：五个hub基因（PIK3R1，IL1A，CXCR4，ACTN1，PFN1）被鉴定，主要富含肌动蛋白相关的生物过程和途径。转录因子RELA,JUN,调节hub基因的NFKB1和FOS调节IL1A表达，而其他21种转录因子调控CXCR4的表达。结论：总之，这项研究可能为CF伴NTM感染的机制提供新的见解。
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