关键词: capillary electrophoresis chromatography fingerprinting isolation mass spectrometry

Mesh : Electrophoresis, Capillary Mass Spectrometry Humans Carbohydrates / chemistry Chromatography, Liquid Hydrophobic and Hydrophilic Interactions

来  源:   DOI:10.1002/jssc.202300668

Abstract:
Saccharides and biocompounds as saccharide (sugar) complexes have various roles and biological functions in living organisms due to modifications via nucleophilic substitution, polymerization, and complex formation reactions. Mostly, mono-, di-, oligo-, and polysaccharides are stabilized to inactive glycosides, which are formed in metabolic pathways. Natural saccharides are important in food and environmental monitoring. Glycosides with various functionalities are significant in clinical and medical research. Saccharides are often studied with the chromatographic methods of hydrophilic interaction liquid chromatography and anion exchange chromatograpy, but also with capillary electrophoresis and mass spectrometry with their on-line coupling systems. Sample preparation is important in the identification of saccharide compounds. The cases discussed here focus on bioscience, clinical, and food applications.
摘要:
由于通过亲核取代进行修饰,糖类和生物化合物作为糖(糖)复合物在活生物体中具有各种作用和生物学功能,聚合,和复杂的形成反应。大多数情况下,mono-,di-,寡头-,多糖被稳定成非活性的糖苷,它们是在代谢途径中形成的。天然糖类在食品和环境监测中具有重要意义。具有各种功能的糖苷在临床和医学研究中具有重要意义。糖类常用亲水作用液相色谱和阴离子交换色谱等色谱方法研究,还有毛细管电泳和质谱及其在线耦合系统。样品制备在糖类化合物的鉴定中很重要。这里讨论的案例集中在生物科学上,临床,和食品应用。
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