Abstract：
A stop-gain mutation (rs715966442; BTA11: 1,02,463,944 nucleotide position) in transcription termination factor, RNA polymerase I (TTF1) gene causes abortion in Holstein Friesian (HF) cattle. A PCR-restriction fragment length polymorphism (PCR-RFLP)-based genetic test has been developed and validated to screen the TTF1 mutation locus in HF cattle. The mutation locus was screened in 80 HF and HF crossbreds using the protocol, which revealed two animals as carriers of the mutant TTF1 allele. The test employed is cost-effective, rapid and precise and can be utilized as an effective tool for the screening of TTF1 mutation carriers in HF cattle population.
摘要：
转录终止因子中的停止增益突变（rs715966442;BTA11：1,02,463,944核苷酸位置），RNA聚合酶I（TTF1）基因导致荷斯坦弗里斯（HF）牛流产。已开发并验证了基于PCR限制性片段长度多态性（PCR-RFLP）的遗传测试，以筛选HF牛的TTF1突变位点。使用该方案在80个HF和HF杂种中筛选了突变基因座，揭示了两只动物是突变TTF1等位基因的携带者。采用的测试具有成本效益，快速，准确，可用作筛选HF牛群中TTF1突变携带者的有效工具。
