Abstract：
We describe a microwave-assisted, methanol and acetic acid-free, inexpensive method for rapid staining of SDS-PAGE proteins. Only citric acid, benzoic acid, and Coomassie brilliant blue G-250 (CBG) were used. Microwave irradiation reduced the detection duration, and proteins in a clear background were visualized within 30 min of destaining, after 2 min of fixing and 12 min of staining. By using this protocol, comparable band intensities were obtained to the conventional methanol/acetic acid method.
摘要：
我们描述了一种微波辅助，不含甲醇和乙酸，廉价的SDS-PAGE蛋白快速染色方法。只有柠檬酸，苯甲酸,使用考马斯亮蓝G-250(CBG)。微波照射缩短了检测时间,和蛋白质在清晰的背景下在30分钟内显示出来，固定2分钟和染色12分钟后。通过使用这个协议，获得了与常规甲醇/乙酸方法相当的谱带强度。
