%0 Journal Article
%T Microwave-assisted and methanol/acetic acid-free method for rapid staining of proteins in SDS-PAGE gels.
%A Wang J
%A Wang Z
%A Zhao W
%A Wang Y
%J Anal Biochem
%V 691
%N 0
%D 2024 Aug 1
%M 38697592
%F 3.191
%R 10.1016/j.ab.2024.115553
%X We describe a microwave-assisted, methanol and acetic acid-free, inexpensive method for rapid staining of SDS-PAGE proteins. Only citric acid, benzoic acid, and Coomassie brilliant blue G-250 (CBG) were used. Microwave irradiation reduced the detection duration, and proteins in a clear background were visualized within 30 min of destaining, after 2 min of fixing and 12 min of staining. By using this protocol, comparable band intensities were obtained to the conventional methanol/acetic acid method.