Abstract：
Cystic echinococcosis is a zoonotic disease resulting from infection caused by the larval stage of Echinococcus granulosus. This study aimed to assess the specific proteins that are potential candidates for the development of a vaccine against E. granulosus. The data-independent acquisition approach was employed to identify differentially expressed proteins (DEPs) in E. granulosus samples. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was employed to identify several noteworthy proteins. Results: The DEPs in E. granulosus samples were identified (245 pericystic wall vs. parasite-free yellowish granuloma (PYG, 1725 PY vs. PYG, 2274 PN vs. PYG). Further examination of these distinct proteins revealed their predominant enrichment in metabolic pathways, amyotrophic lateral sclerosis, and neurodegeneration-associated pathways. Notably, among these DEPs, SH3BGRL, MST1, TAGLN2, FABP5, UBE2V2, and RARRES2 exhibited significantly higher expression levels in the PYG group compared with the PY group (P < 0.05). The findings may contribute to the understanding of the pathological mechanisms underlying echinococcosis, providing valuable insights into the development of more effective diagnostic tools, treatment modalities, and preventive strategies. SIGNIFICANCE: CE is a major public health hazard in the western regions of China, Central Asia, South America, the Mediterranean countries, and eastern Africa. Echinococcus granulosus is responsible for zoonotic disease through infection Our analysis focuses on the proteins in various samples by data-dependent acquisition (DIA) for proteomic analysis. The importance of this research is to develop new strategies and targets to protect against E. granulosus infections in humans.
摘要：
囊性包虫病是一种由细粒棘球蚴幼虫期感染引起的人畜共患疾病。这项研究旨在评估特定的蛋白质，这些蛋白质是开发针对E.granulosus的疫苗的潜在候选物。采用独立于数据的采集方法来鉴定颗粒大肠杆菌样品中差异表达的蛋白质(DEP)。采用基因本体论(GO)和京都基因和基因组百科全书(KEGG)途径富集分析来鉴定几种值得注意的蛋白质。结果：确定了E.granulosus样品中的DEP（245周囊壁与无寄生虫黄色肉芽肿（PYG，1725PYvs.PYG,2274PNvs.PYG）。对这些不同蛋白质的进一步检查揭示了它们在代谢途径中的主要富集，肌萎缩侧索硬化，和神经变性相关通路。值得注意的是,在这些DEP中,SH3BGRL,MST1、TAGLN2、FABP5、UBE2V2和RARRES2在PYG组的表达水平明显高于PY组(P<0.05)。这些发现可能有助于理解包虫病的病理机制。为开发更有效的诊断工具提供有价值的见解，治疗方式，和预防策略。意义：CE是中国西部地区的主要公共卫生危害，中亚,南美洲,地中海国家,和东部非洲。细粒棘球蚴是通过感染引起人畜共患疾病的原因我们的分析重点是通过数据依赖性采集（DIA）进行蛋白质组学分析的各种样品中的蛋白质。这项研究的重要性是开发新的策略和目标，以防止人类感染。
