Abstract：
In newly commissioned drinking-water polyethylene (PE) pipes, biofilm develops on the inner pipe surface. The microbial community composition from colonization to the establishment of mature biofilms is less known, including the effect on the distributed water quality. Biofilm development was followed through 1.5 years in PE-pipe side streams at two locations of a full-scale, non-chlorinated drinking-water distribution system (leaving a waterworks versus 5-6 km from a waterworks) along with inlet and outlet water quality. Mature biofilms were established after ∼8-9 months, dominated by Proteobacteria, Actinobacteria and Saccharibacteria (61-93% relative abundance), with a higher diversity (OTUs/Shannon Index/16S rRNA gene amplicon sequencing) in pipes in the far end of the distribution system. Comamonadaceae, and specifically Aquabacterium (>30% of reads), dominated young (∼1.5-month-old) biofilms. Young biofilms were linked to increased microbiological counts in drinking water (HPC/ATP/qPCR), while the establishment of mature biofilms led to a drop in HPC and benefited the water quality, highlighting the importance of optimizing commissioning procedures for rapidly achieving mature and stable biofilms.
摘要：
在新调试的饮用水聚乙烯（PE）管道中，生物膜在内管表面发展。从定殖到建立成熟生物膜的微生物群落组成鲜为人知，包括对分布式水质的影响。生物膜的发展经过了1.5年的PE管侧流在两个位置的全面，非氯化饮用水分配系统（离开自来水厂而离开自来水厂5-6公里）以及入口和出口水质。成熟的生物膜在8-9个月后建立，以变形杆菌为主，放线菌和酵母菌（61-93%相对丰度），在分配系统的远端管道中具有更高的多样性（OTU/ShannonIndex/16SrRNA基因扩增子测序）。Comamonadaceae,特别是水细菌(>30%的读数)，占主导地位的年轻(~1.5个月大)生物膜。年轻的生物膜与饮用水中微生物计数增加有关（HPC/ATP/qPCR），虽然成熟生物膜的建立导致HPC下降，并有利于水质，强调优化调试程序以快速实现成熟和稳定的生物膜的重要性。
