PDF(Pubmed)
Abstract：
Stem cells (SCs) have been used therapeutically for decades, yet their applications are limited by factors such as the risk of immune rejection and potential tumorigenicity. Extracellular vesicles (EVs), a key paracrine component of stem cell potency, overcome the drawbacks of stem cell applications as a cell-free therapeutic agent and play an important role in treating various diseases. However, EVs derived from two-dimensional (2D) planar culture of SCs have low yield and face challenges in large-scale production, which hinders the clinical translation of EVs. Three-dimensional (3D) culture, given its ability to more realistically simulate the in vivo environment, can not only expand SCs in large quantities, but also improve the yield and activity of EVs, changing the content of EVs and improving their therapeutic effects. In this review, we briefly describe the advantages of EVs and EV-related clinical applications, provide an overview of 3D cell culture, and finally focus on specific applications and future perspectives of EVs derived from 3D culture of different SCs.
摘要：
干细胞（SCs）已经在治疗上使用了几十年，然而，它们的应用受到免疫排斥风险和潜在致瘤性等因素的限制。细胞外囊泡（EV），干细胞潜能的关键旁分泌成分，克服了干细胞作为无细胞治疗剂应用的缺点，在治疗各种疾病中发挥着重要作用。然而,从二维（2D）平面培养的SC衍生的EV产量低，在大规模生产中面临挑战。这阻碍了电动汽车的临床翻译。三维（3D）文化，鉴于它能够更真实地模拟体内环境，不仅可以大量扩增SC，还可以提高电动汽车的产量和活性，改变电动汽车的含量，提高其治疗效果。在这次审查中,我们简要介绍了电动汽车和电动汽车相关临床应用的优势，提供3D细胞培养的概述，最后重点介绍了不同SC3D培养的电动汽车的具体应用和未来展望。
