RESULTS: In order to understand the biological function of the WOX gene family in blueberries, bioinformatics were used methods to identify WOX gene family members in the blueberry genome, and analyzed the basic physical and chemical properties, gene structure, gene motifs, promoter cis-acting elements, chromosome location, evolutionary relationships, expression pattern of these family members and predicted their functions. Finally, 12 genes containing the WOX domain were identified and found to be distributed on eight chromosomes. Phylogenetic tree analysis showed that the blueberry WOX gene family had three major branches: ancient branch, middle branch, and WUS branch. Blueberry WOX gene family protein sequences differ in amino acid number, molecular weight, isoelectric point and hydrophobicity. Predictive analysis of promoter cis-acting elements showed that the promoters of the VdWOX genes contained abundant light response, hormone, and stress response elements. The VdWOX genes were induced to express in both stems and leaves in response to salt and drought stress.
CONCLUSIONS: Our results provided comprehensive characteristics of the WOX gene family and important clues for further exploration of its role in the growth, development and resistance to various stress in blueberry plants.
结果:为了了解蓝莓中WOX基因家族的生物学功能,利用生物信息学方法鉴定蓝莓基因组中WOX基因家族成员,并分析了基本的物理和化学性质,基因结构,基因基序,启动子顺式作用元件,染色体位置,进化关系,这些家族成员的表达模式并预测其功能。最后,鉴定出12个含有WOX结构域的基因,发现它们分布在8条染色体上。系统发育树分析表明,蓝莓WOX基因家族有三个主要分支:古代分支,中间分支,和WUS分支。蓝莓WOX基因家族蛋白质序列的氨基酸数目不同,分子量,等电点和疏水性。对启动子顺式作用元件的预测分析表明,VdWOX基因的启动子含有丰富的光响应,激素,和应激反应元素。响应于盐和干旱胁迫,VdWOX基因被诱导在茎和叶中表达。
结论:我们的结果提供了WOX基因家族的综合特征,为进一步探索其在生长中的作用提供了重要线索。蓝莓植物的发育和对各种胁迫的抗性。