Abstract：
Serodiagnosis methods have been used as platforms for diagnostic tests for many diseases. Due to magnetic nanoparticles\' properties to quickly detach from an external magnetic field and particle size effects, these nanomaterials\' functionalization allows the specific isolation of target analytes, enhancing accuracy parameters and reducing serodiagnosis time. Superparamagnetic iron oxide nanoparticles (MNPs) were synthesized and functionalized with polyethylene glycol (PEG) and then associated with the synthetic Leishmaniosis epitope. This nano-peptide antigen showed promising results. Regarding Tegumentary leishmaniasis diagnostic accuracy, the AUC was 0.8398 with sensibility 75% (95CI% 50.50 - 89.82) and specificity 87.50% (95CI% 71.93 - 95.03), and Visceral leishmaniasis accuracy study also present high performance, the AUC was 0.9258 with sensibility 87.50% (95CI% 63.98 - 97.78) and specificity 87.50% (95CI% 71.93 - 95.03). Our results demonstrate that the association of the antigen with MNPs accelerates and improves the diagnosis process. MNPs could be an important tool for enhancing serodiagnosis.
摘要：
血清诊断方法已被用作许多疾病的诊断测试的平台。由于磁性纳米粒子的特性可以快速脱离外部磁场和粒径效应，这些纳米材料的功能化允许目标分析物的特定分离，提高准确性参数和减少血清诊断时间。合成了超顺磁性氧化铁纳米颗粒（MNPs），并用聚乙二醇（PEG）官能化，然后与合成的利什曼病表位结合。该纳米肽抗原显示有希望的结果。关于肿瘤利什曼病的诊断准确性，AUC为0.8398，敏感性为75％（95CI％50.50-89.82），特异性为87.50％（95CI％71.93-95.03），和内脏利什曼病的准确性研究也呈现出很高的性能，AUC为0.9258,敏感性为87.50%(95CI%63.98-97.78),特异性为87.50%(95CI%71.93-95.03).我们的结果表明，抗原与MNPs的结合加速并改善了诊断过程。MNPs可能是增强血清诊断的重要工具。
