PDF(Pubmed)
Abstract：
UNASSIGNED: To optimize the multiplex polymerase chain reaction (M-PCR) technique to diagnose microdeletions of azoospermia factors (AZF) on the Y chromosome and initially apply the technique to diagnose male patients with sperm density less than 5×106 million sperm/mL was assigned to do a test to check for AZF microdeletions on the Y chromosome.
UNASSIGNED: Based on the positive control samples which belong to male subjects who have had 2 healthy children without any assisted reproductive technologies, the M-PCR method was developed to detect simultaneously and accurately AZF microdeletions on 32 male patients with sperm densities below 5×106 million sperm/mL of semen at the Department of Biology and Medical Genetics - Vietnam Military Medical University.
UNASSIGNED: Successful optimization of the M-PCR technique including 7 reactions arranged according to each AZFabc region using 24 STS/gene on the Y chromosome. Initial application to diagnose AZF deletion on 32 azoospermic and oligospermic men reveals that AZFa deletion accounts for 6.25% (2/32); deletion of all 3 regions AZFa,b,c with 18.75% (6/32 cases); The combined deletion rate of AZFb,c is highest, accounting for 56.24% (18/32 patients).
UNASSIGNED: Successfully optimized the M-PCR technique in identifying AZF microdeletions using 24 sequence tagged sites (STS)/gene for azoospermic and oligozoospermic men. The M-PCR technique has great potential in the application of AZF deletion diagnosis.
UNASSIGNED: Based on the positive control samples which belong to male subjects who have had 2 healthy children without any assisted reproductive technologies, the M-PCR method was developed to detect simultaneously and accurately AZF microdeletions on 32 male patients with sperm densities below 5×106 million sperm/mL of semen at the Department of Biology and Medical Genetics - Vietnam Military Medical University.
UNASSIGNED: Successful optimization of the M-PCR technique including 7 reactions arranged according to each AZFabc region using 24 STS/gene on the Y chromosome. Initial application to diagnose AZF deletion on 32 azoospermic and oligospermic men reveals that AZFa deletion accounts for 6.25% (2/32); deletion of all 3 regions AZFa,b,c with 18.75% (6/32 cases); The combined deletion rate of AZFb,c is highest, accounting for 56.24% (18/32 patients).
UNASSIGNED: Successfully optimized the M-PCR technique in identifying AZF microdeletions using 24 sequence tagged sites (STS)/gene for azoospermic and oligozoospermic men. The M-PCR technique has great potential in the application of AZF deletion diagnosis.
摘要：
■优化多重聚合酶链反应（M-PCR）技术以诊断Y染色体上无精子症因子（AZF）的微缺失，并初步应用该技术诊断精子密度小于5×1.06亿精子/mL的男性患者进行测试，以检查Y染色体上的AZF微缺失。
■基于阳性对照样本，这些样本属于男性受试者，他们有2个健康孩子，没有任何辅助生殖技术，在越南军医大学生物学和医学遗传学系，开发了M-PCR方法，以同时准确地检测32名精子密度低于5×1.06亿精子/mL精液的男性患者的AZF微缺失。
■M-PCR技术的成功优化，包括使用Y染色体上的24个STS/基因根据每个AZFabc区域排列的7个反应。对32名无精子和少精子男性的AZF缺失进行初步诊断，发现AZFa缺失占6.25％（2/32）；所有3个区域AZFa的缺失，B,c占18.75%(6/32例)；AZFb的合并缺失率，C最高,占56.24%(18/32例)。
■使用24个序列标记位点（STS）/基因成功优化了M-PCR技术，以鉴定无精子症和少精子症男性的AZF微缺失。M-PCR技术在AZF缺失诊断中具有很大的应用潜力。
■基于阳性对照样本，这些样本属于男性受试者，他们有2个健康孩子，没有任何辅助生殖技术，在越南军医大学生物学和医学遗传学系，开发了M-PCR方法，以同时准确地检测32名精子密度低于5×1.06亿精子/mL精液的男性患者的AZF微缺失。
■M-PCR技术的成功优化，包括使用Y染色体上的24个STS/基因根据每个AZFabc区域排列的7个反应。对32名无精子和少精子男性的AZF缺失进行初步诊断，发现AZFa缺失占6.25％（2/32）；所有3个区域AZFa的缺失，B,c占18.75%(6/32例)；AZFb的合并缺失率，C最高,占56.24%(18/32例)。
■使用24个序列标记位点（STS）/基因成功优化了M-PCR技术，以鉴定无精子症和少精子症男性的AZF微缺失。M-PCR技术在AZF缺失诊断中具有很大的应用潜力。
