{Reference Type}: Journal Article
{Title}: Optimization of Multiplex-PCR Technique To Determine Azf Deletions in infertility Male Patients.
{Author}: Thanh TN;Tien ST;Van PN;Thai SD;Cong TL;Le TD;Nguyen ST;Van TT;Duong HH;Bui TM;Nguyen KT;
{Journal}: Int J Gen Med
{Volume}: 17
{Issue}: 0
{Year}: 2024
{Factor}: 2.145
{DOI}: 10.2147/IJGM.S455513
{Abstract}: <B>UNASSIGNED: </B>To optimize the multiplex polymerase chain reaction (M-PCR) technique to diagnose microdeletions of azoospermia factors (AZF) on the Y chromosome and initially apply the technique to diagnose male patients with sperm density less than 5×106 million sperm/mL was assigned to do a test to check for AZF microdeletions on the Y chromosome.<BR><B>UNASSIGNED: </B>Based on the positive control samples which belong to male subjects who have had 2 healthy children without any assisted reproductive technologies, the M-PCR method was developed to detect simultaneously and accurately AZF microdeletions on 32 male patients with sperm densities below 5×106 million sperm/mL of semen at the Department of Biology and Medical Genetics - Vietnam Military Medical University.<BR><B>UNASSIGNED: </B>Successful optimization of the M-PCR technique including 7 reactions arranged according to each AZFabc region using 24 STS/gene on the Y chromosome. Initial application to diagnose AZF deletion on 32 azoospermic and oligospermic men reveals that AZFa deletion accounts for 6.25% (2/32); deletion of all 3 regions AZFa,b,c with 18.75% (6/32 cases); The combined deletion rate of AZFb,c is highest, accounting for 56.24% (18/32 patients).<BR><B>UNASSIGNED: </B>Successfully optimized the M-PCR technique in identifying AZF microdeletions using 24 sequence tagged sites (STS)/gene for azoospermic and oligozoospermic men. The M-PCR technique has great potential in the application of AZF deletion diagnosis.