PDF(Pubmed)
Abstract：
In recent decades, an enormous potential of fungal-based products with characteristics equal to, or even outperforming, classic petroleum-derived products has been acknowledged. The production of these new materials uses mycelium, a root-like structure of fungi consisting of a mass of branching, thread-like hyphae. Optimizing the production of mycelium-based materials and fungal growth under technical conditions needs to be further investigated. The main objective of this study was to select fast-growing fungi and identify optimized incubation conditions to obtain a dense mycelium mat in a short time. Further, the influence of the initial substrate characteristics on hyphae expansion was determined. Fungal isolates of Ganoderma lucidum, Pleurotus ostreatus, and Trametes versicolor were cultivated for seven days on substrate mixtures consisting of various proportions of pine bark and cotton fibers. Furthermore, the substrates were mixed with 0, 2, and 5 wt.% calcium carbonate (CaCO3), and the incubator was flushed with 0, 5, and 10 vol.% carbon dioxide (CO2). All samples grew in the dark at 26 °C and a relative humidity of 80%. Evaluation of growth rate shows that cotton fiber-rich substrates performed best for all investigated fungi. Although Pleurotus ostreatus and Trametes versicolor showed comparatively high growth rates of up to 5.4 and 5.3 mm d-1, respectively, mycelium density was thin and transparent. Ganoderma lucidum showed a significantly denser mycelium at a maximum growth rate of 3.3 mm d-1 on a cotton fiber-rich substrate (75 wt.%) without CaCO3 but flushed with 5 vol.% CO2 during incubation.
摘要：
近几十年来,以真菌为基础的产品具有巨大的潜力，甚至表现优异，经典的石油衍生产品已得到认可。这些新材料的生产使用菌丝体，真菌的根状结构，由大量的分支组成，线状菌丝.在技术条件下优化菌丝体材料的生产和真菌的生长需要进一步研究。本研究的主要目的是选择快速生长的真菌并确定优化的孵育条件，以在短时间内获得致密的菌丝体垫。Further,确定了初始底物特征对菌丝扩张的影响。灵芝真菌分离株,平菇,和杂色Trametes在由各种比例的松树皮和棉纤维组成的基质混合物上培养7天。此外，将底物与0、2和5重量%混合。%碳酸钙(CaCO3)，用0、5和10体积的培养箱冲洗。%二氧化碳(CO2)。所有样品在26°C和80%的相对湿度下在黑暗中生长。生长速率的评估表明，富含棉纤维的基质对于所有研究的真菌都表现最好。尽管平菇和杂色平菇表现出相对较高的生长速率，分别高达5.4和5.3mmd-1，菌丝密度薄而透明。灵芝在富含棉纤维的基质上以3.3mmd-1的最大生长速率显示出明显致密的菌丝体（75wt.%)不含CaCO3，但用5vol冲洗。孵育期间的%CO2。
