Abstract：
Brain edema causes abnormal fluid retention and can be fatal in severe cases. Although it develops in various diseases, most treatments for brain edema are classical. We analyzed the impacts of age and gender on the characteristics of a water intoxication model that induces pure brain edema in mice and examined the model\'s usefulness for research regarding new treatments for brain edema. C57BL/6J mice received an intraperitoneal administration of 10% body weight distilled water, and we calculated the brain water content by measuring the brain-tissue weight immediately after dissection and after drying. We analyzed 8-OHdG and caspase-3 values to investigate the brain damage. We also applied this model in aquaporin 4 knockout (AQP4-) mice and compared these mice with wild-type mice. The changes in water content differed by age and gender, and the 8-OHdG and caspase-3 values differed by age. Suppression of brain edema by AQP4- was also confirmed. These results clarified the differences in the onset of brain edema by age and gender, highlighting the importance of considering the age and gender of model animals. Similar studies using genetically modified mice are also possible. Our findings indicate that this water intoxication model is effective for explorations of new brain edema treatments.
摘要：
脑水肿会导致异常的液体潴留，在严重的情况下可能是致命的。虽然它在各种疾病中发展，大多数治疗脑水肿的方法都是经典的。我们分析了年龄和性别对诱导小鼠纯脑水肿的水中毒模型特征的影响，并研究了该模型对脑水肿新疗法研究的有用性。C57BL/6J小鼠腹腔注射10%体重的蒸馏水，我们通过在解剖和干燥后立即测量脑组织重量来计算脑含水量。我们分析了8-OHdG和caspase-3值以研究脑损伤。我们还在水通道蛋白4敲除（AQP4-）小鼠中应用了该模型，并将这些小鼠与野生型小鼠进行了比较。含水量的变化因年龄和性别而异，8-OHdG和caspase-3值因年龄而异。还证实了AQP4-对脑水肿的抑制。这些结果阐明了不同年龄和性别的脑水肿发作的差异,强调考虑模型动物的年龄和性别的重要性。使用转基因小鼠的类似研究也是可能的。我们的发现表明，这种水中毒模型对于探索新的脑水肿治疗方法是有效的。
