PDF(Pubmed)
Abstract：
In laying hens, fatty liver hemorrhagic syndrome (FLHS) is a common metabolic disorder, which can affect egg production and nutritional value. However, the impact of FLHS on the lipid content in egg yolks was not clear. In this study, FLHS model was induced by using high-energy low-protein diet, and the egg quality was evaluated. Egg yolk lipids were quantitatively analyzed by using ultra-performance liquid chromatography-mass spectrometry combined with multivariate statistical analysis. Gene expressions of the lipoprotein were determined by qRT-PCR and antioxidant capacity of the egg yolk were determined by kits. The elevated blood lipids and extensive lipid droplets observed indicated successful establishment of the FLHS model in laying hens. Measurements of egg quality showed that egg yolk weight was increased in the FLHS group. Lipidomics revealed that 1,401 lipids, comprising 27 lipid subclasses in the egg yolk. According to score plots of principal component analysis and orthogonal partial least squares discriminant analysis, different lipid profile was observed between the control and FLHS groups. A total of 97 different lipid species were screen out. Sphingolipid and glycerophospholipid metabolism were identified as key pathways. Free polyunsaturated fatty acids (PUFA) exhibited an increase in the FLHS group (P < 0.05). Notably, the form of PUFAs was changed that the FLHS group showed an increase in triacylglycerol-docosahexenoic acid and triacylglycerol-arachidonic acid in the egg yolk, while triacylglycerol-α-linolenic acid was decreased (P < 0.05). Total superoxide dismutase was decreased in the egg yolks affected by FLHS. Gene expressions of vitellogenin 2 (VTG2), VTG3, very low-density apolipoprotein II and apolipoprotein B were increased in the liver of laying hens with FLHS (P < 0.05). In conclusion, FLHS promoted the lipid transport from the liver to the yolk by upregulating lipoprotein expression, which altered lipid profile, and reduced antioxidant capacity in the yolk. This study provided a foundation for understanding the changes in lipids, lipid transport and lipid antioxidation capacity in egg yolk from laying hens with FLHS.
摘要：
在产蛋鸡中，脂肪肝出血性综合征(FLHS)是一种常见的代谢紊乱,会影响鸡蛋的产量和营养价值。然而,FLHS对蛋黄脂质含量的影响尚不清楚。在这项研究中,采用高能低蛋白饮食诱导FLHS模型,并对鸡蛋质量进行了评价。采用超高效液相色谱-质谱联用技术结合多元统计分析对蛋黄脂质进行定量分析。通过qRT-PCR测定脂蛋白的基因表达，并通过试剂盒测定蛋黄的抗氧化能力。观察到的血脂升高和广泛的脂滴表明在蛋鸡中成功建立了FLHS模型。鸡蛋质量的测量表明，FLHS组蛋黄重量增加。脂质组学显示，1,401脂质，在蛋黄中包含27个脂质亚类。根据主成分分析和正交偏最小二乘判别分析的得分图，对照组和FLHS组之间观察到不同的血脂特征.总共筛选出97种不同的脂质种类。鞘脂和甘油磷脂代谢被确定为关键途径。游离多不饱和脂肪酸(PUFA)在FLHS组增加(P<0.05)。值得注意的是,PUFA的形式发生了变化，FLHS组显示蛋黄中三酰甘油-二十二碳六烯酸和三酰甘油-花生四烯酸的增加，三酰甘油-α-亚麻酸降低(P<0.05)。受FLHS影响的蛋黄中总超氧化物歧化酶降低。卵黄蛋白原2(VTG2)基因表达,FLHS蛋鸡肝脏中VTG3，极低密度载脂蛋白II和载脂蛋白B升高（P<0.05）。总之,FLHS通过上调脂蛋白表达促进从肝脏到卵黄的脂质转运，改变了血脂分布，蛋黄中的抗氧化能力降低。这项研究为了解脂质的变化提供了基础，FLHS蛋鸡蛋黄的脂质转运和脂质抗氧化能力。
