Abstract：
The Bangkaew dog is an indigenous dog breed in the Phitsanulok province of Thailand. This breed is recognized by the Fédération Cynologique Internationale (FCI), a global canine organization. The unique traits of the Bangkaew breed lead to purebred selection for breeding, while only their traits and pedigree from parental history are recorded. Determination of the risk of inbreeding depression and the origin of unknown DNA profiles is essential due to the challenges in predicting puppy characteristics, which are crucial for breed management and conservation.
This study aimed to emphasize that current allelic frequency data for the Bangkaew dog breed must be considered for precise individual identification.
Approximately 82 Bangkaew dogs from various Thai localities were studied using 15 microsatellite markers for genotypic monitoring and individual identification. Maternal genetic inheritance was assessed via mtDNA D-loop analysis.
The results revealed high genetic diversity in the Bangkaew breed, indicating low potential for inbreeding. We also found that using a 15 loci microsatellite panel was effective for the identification of Bangkaew dogs. The optimized 10 loci microsatellite genotyping panel developed in this study presents improved identification testing efficiency, promoting both time- and cost-effectiveness.
Analysis of microsatellite DNA markers in Bangkaew dogs using an optimized panel of 10 loci selected from 15 loci effectively facilitated individual identification. This approach not only enhances time and cost efficiency, but also provides accurate allelic frequency estimates, which are crucial for the realistic evaluation of DNA evidence.
This study aimed to emphasize that current allelic frequency data for the Bangkaew dog breed must be considered for precise individual identification.
Approximately 82 Bangkaew dogs from various Thai localities were studied using 15 microsatellite markers for genotypic monitoring and individual identification. Maternal genetic inheritance was assessed via mtDNA D-loop analysis.
The results revealed high genetic diversity in the Bangkaew breed, indicating low potential for inbreeding. We also found that using a 15 loci microsatellite panel was effective for the identification of Bangkaew dogs. The optimized 10 loci microsatellite genotyping panel developed in this study presents improved identification testing efficiency, promoting both time- and cost-effectiveness.
Analysis of microsatellite DNA markers in Bangkaew dogs using an optimized panel of 10 loci selected from 15 loci effectively facilitated individual identification. This approach not only enhances time and cost efficiency, but also provides accurate allelic frequency estimates, which are crucial for the realistic evaluation of DNA evidence.
摘要：
背景：Bangkaew狗是泰国彭萨努洛省的一种本土狗品种。该品种得到国际食环学家联合会（FCI）的认可，一个全球性的犬类组织.Bangkaew品种的独特性状导致纯种育种选择，而仅记录了父母历史中的特征和谱系。由于预测幼犬特征的挑战，因此确定近亲繁殖抑郁症的风险和未知DNA谱的起源至关重要。这对品种管理和保护至关重要。
目的：这项研究旨在强调，必须考虑Bangkaew犬品种的当前等位基因频率数据以进行精确的个体鉴定。
方法：使用15个微卫星标记研究了来自泰国各地的大约82只Bangkaew犬，用于基因型监测和个体鉴定。通过mtDNAD-loop分析评估母体遗传遗传。
结果：结果显示Bangkaew品种具有很高的遗传多样性，表明近亲繁殖的可能性很低。我们还发现，使用15个基因座的微卫星面板可有效识别Bangkaew犬。本研究中开发的优化的10个基因座微卫星基因分型小组提出了改进的鉴定测试效率，促进时间和成本效益。
结论：使用从15个基因座中选择的10个基因座的优化组，对Bangkaew犬的微卫星DNA标记进行分析，有效地促进了个体鉴定。这种方法不仅提高了时间和成本效率，而且还提供了准确的等位基因频率估计，这对于DNA证据的现实评估至关重要。
目的：这项研究旨在强调，必须考虑Bangkaew犬品种的当前等位基因频率数据以进行精确的个体鉴定。
方法：使用15个微卫星标记研究了来自泰国各地的大约82只Bangkaew犬，用于基因型监测和个体鉴定。通过mtDNAD-loop分析评估母体遗传遗传。
结果：结果显示Bangkaew品种具有很高的遗传多样性，表明近亲繁殖的可能性很低。我们还发现，使用15个基因座的微卫星面板可有效识别Bangkaew犬。本研究中开发的优化的10个基因座微卫星基因分型小组提出了改进的鉴定测试效率，促进时间和成本效益。
结论：使用从15个基因座中选择的10个基因座的优化组，对Bangkaew犬的微卫星DNA标记进行分析，有效地促进了个体鉴定。这种方法不仅提高了时间和成本效率，而且还提供了准确的等位基因频率估计，这对于DNA证据的现实评估至关重要。
