PDF(Pubmed)
Abstract：
An investigation into retrovirus was conducted in six species of bats (Myotis aurascens, Myotis petax, Myotis macrodactylus, Miniopterus fuliginosus, Rhinolophus ferrumequinum, and Pipistrellus abramus) inhabiting South Korea. Exogenous retroviruses (XRVs) were detected in the tissue samples of R. ferrumequinum individuals by PCR assay. Proviruses were identified in all tissue samples through viral quantification using a digital PCR assay per organ (lung, intestine, heart, brain, wing, kidney, and liver), with viral loads varying greatly between each organ. In phylogenetic analysis based on the whole genome, the Korean bat retroviruses and the R. ferrumequinum retrovirus (RfRV) strain formed a new clade distinct from the Gammaretrovirus clade. The phylogenetic results determined these viruses to be RfRV-like viruses. In the Simplot comparison, Korean RfRV-like viruses exhibited relatively strong fluctuated patterns in the latter part of the envelope gene area compared to other gene areas. Several point mutations within this region (6,878-7,774 bp) of these viruses were observed compared to the RfRV sequence. One Korean RfRV-like virus (named Y4b strain) was successfully recovered in the Raw 264.7 cell line, and virus particles replicated in the cells were confirmed by transmission electron microscopy. RfRVs (or RfRV-like viruses) have been spreading since their first discovery in 2012, and the Korean RfRV-like viruses were assumed to be XRVs that evolved from RfRV.IMPORTANCER. ferrumequinum retrovirus (RfRV)-like viruses were identified in greater horseshoe bats in South Korea. These RfRV-like viruses were considered exogenous retroviruses (XRVs) that emerged from RfRV. Varying amounts of provirus detected in different organs suggest ongoing viral activity, replication, and de novo integration in certain organs. Additionally, the successful recovery of the virus in the Raw 264.7 cell line provides strong evidence supporting their status as XRVs. These viruses have now been identified in South Korea and, more recently, in Kenya since RfRV was discovered in China in 2012, indicating that RfRVs (or RfRV-like viruses) have spread worldwide.
摘要：
在六种蝙蝠（Myotisaurascens，Myotispetax，大肌炎,小翅目，费美喹犀牛,和Pipistrelusabramus）居住在韩国。通过PCR测定在R.ferrumequinum个体的组织样品中检测到外源逆转录病毒（XRV）。通过使用每个器官的数字PCR测定法进行病毒定量，在所有组织样品中鉴定出了病毒（肺，肠,心,大脑,机翼,肾,和肝脏)，每个器官之间的病毒载量差异很大。在基于全基因组的系统发育分析中，韩国蝙蝠逆转录病毒和R.ferrumequinum逆转录病毒（RfRV）毒株形成了与Gammadetrovirus进化枝不同的新进化枝。系统发育结果确定这些病毒是RfRV样病毒。在Simplot比较中，与其他基因区域相比，韩国RfRV样病毒在包膜基因区域的后半部分表现出相对强烈的波动模式。与RfRV序列相比,观察到这些病毒的该区域内的几个点突变(6,878-7,774bp)。在Raw264.7细胞系中成功回收了一种韩国RfRV样病毒（命名为Y4b株），通过透射电子显微镜证实了细胞中复制的病毒颗粒。RfRV（或RfRV样病毒）自2012年首次发现以来一直在传播，韩国RfRV样病毒被认为是从RfRV进化而来的XRV。IMPORTANCER.在韩国的大型马蹄形蝙蝠中发现了类似费美喹逆转录病毒（RfRV）的病毒。这些RfRV样病毒被认为是从RfRV出现的外源性逆转录病毒(XRV)。在不同器官中检测到的不同数量的前病毒表明持续的病毒活性，复制,在某些器官中从头整合。此外,Raw264.7细胞系中病毒的成功回收提供了强有力的证据支持其作为XRV的地位。这些病毒现在已经在韩国被发现，最近,自2012年在中国发现RfRV以来，RfRV在肯尼亚，这表明RfRV（或RfRV样病毒）已在全球范围内传播。
