Abstract：
UNASSIGNED: In order to contribute new insights for future prevention and treatment of intrahepatic cholestasis of pregnancy (ICP), and to promote positive pregnancy outcomes, we evaluated serum Ca2+ levels and inositol 1,4,5-trisphosphate receptor (InsP3R) expression in the liver tissue of a rat ICP model.
UNASSIGNED: After establishing the model by injection of oestradiol benzoate and progesterone into pregnant rats, animals were divided into normal control (n = 5) and ICP model groups (n = 5). The expression of InsP3R protein in the liver, and serum levels of Ca2+, glycocholic acid and bile acid were detected.
UNASSIGNED: InsP3R mRNA and protein were significantly lower in the ICP model group compared to the normal group, as determined by qPCR and immunohistochemistry, respectively. Serum enzyme-linked immunosorbent assay results revealed significantly higher levels of glycocholic acid and bile acid in the ICP model group compared to the normal group, while Ca2+ levels were significantly lower. The levers of Ca2+ were significantly and negatively correlated with the levels of glycocholic acid. The observed decrease in Ca2+ was associated with an increase in total bile acids, but there was no significant correlation.
UNASSIGNED: Our results revealed that the expression of InsP3R and serum Ca2+ levels was significantly decreased in the liver tissue of ICP model rats. Additionally, Ca2+ levels were found to be negatively correlated with the level of glycocholic acid.
This study investigated the relationship between serum Ca2+ levels, inositol 1,4,5-trisphosphate receptor (InsP3R) expression and intrahepatic cholestasis of pregnancy (ICP) in a rat model. The results indicated a significant decrease in InsP3R expression and Ca2+ in the disease group compared to the control group, alongside elevated levels of glycocholic acid and bile acid. The levels of Ca2+ exhibited a negative correlation with the levels of glycocholic acid. These findings indicated that the decrease of InsP3R expression and Ca2+ levels may be related to the pathogenesis of ICP. The study provides further insight into the treatment of this disease.
UNASSIGNED: After establishing the model by injection of oestradiol benzoate and progesterone into pregnant rats, animals were divided into normal control (n = 5) and ICP model groups (n = 5). The expression of InsP3R protein in the liver, and serum levels of Ca2+, glycocholic acid and bile acid were detected.
UNASSIGNED: InsP3R mRNA and protein were significantly lower in the ICP model group compared to the normal group, as determined by qPCR and immunohistochemistry, respectively. Serum enzyme-linked immunosorbent assay results revealed significantly higher levels of glycocholic acid and bile acid in the ICP model group compared to the normal group, while Ca2+ levels were significantly lower. The levers of Ca2+ were significantly and negatively correlated with the levels of glycocholic acid. The observed decrease in Ca2+ was associated with an increase in total bile acids, but there was no significant correlation.
UNASSIGNED: Our results revealed that the expression of InsP3R and serum Ca2+ levels was significantly decreased in the liver tissue of ICP model rats. Additionally, Ca2+ levels were found to be negatively correlated with the level of glycocholic acid.
This study investigated the relationship between serum Ca2+ levels, inositol 1,4,5-trisphosphate receptor (InsP3R) expression and intrahepatic cholestasis of pregnancy (ICP) in a rat model. The results indicated a significant decrease in InsP3R expression and Ca2+ in the disease group compared to the control group, alongside elevated levels of glycocholic acid and bile acid. The levels of Ca2+ exhibited a negative correlation with the levels of glycocholic acid. These findings indicated that the decrease of InsP3R expression and Ca2+ levels may be related to the pathogenesis of ICP. The study provides further insight into the treatment of this disease.
摘要：
■为了为将来预防和治疗妊娠肝内胆汁淤积症（ICP）提供新的见解，并促进积极的怀孕结果，我们评估了大鼠ICP模型肝组织中血清Ca2水平和肌醇1,4,5-三磷酸受体（InsP3R）的表达。
■孕鼠注射苯甲酸雌二醇和孕酮建立模型后，动物分为正常对照组(n=5)和ICP模型组(n=5)。InsP3R蛋白在肝脏中的表达,和血清Ca2+水平，检测甘胆酸和胆汁酸。
■InsP3RmRNA和蛋白在ICP模型组明显低于正常组,通过qPCR和免疫组织化学确定，分别。血清酶联免疫吸附试验结果显示ICP模型组的甘胆酸和胆汁酸水平明显高于正常组,而Ca2+水平明显降低。Ca2+水平与甘氨胆酸水平呈显著负相关。观察到的Ca2+的减少与总胆汁酸的增加有关,但没有显著的相关性。
■我们的结果表明，ICP模型大鼠肝组织中InsP3R的表达和血清Ca2水平显着降低。此外,发现Ca2水平与甘胆酸水平呈负相关。
这项研究调查了血清Ca2+水平之间的关系，肌醇1,4,5-三磷酸受体(InsP3R)表达与妊娠肝内胆汁淤积症(ICP)的关系.结果表明，与对照组相比，疾病组InsP3R表达和Ca2+显著下降，伴随着甘胆酸和胆汁酸水平升高。Ca2水平与甘胆酸水平呈负相关。这些结果表明，InsP3R表达和Ca2水平的降低可能与ICP的发病有关。该研究为这种疾病的治疗提供了进一步的见解。
■孕鼠注射苯甲酸雌二醇和孕酮建立模型后，动物分为正常对照组(n=5)和ICP模型组(n=5)。InsP3R蛋白在肝脏中的表达,和血清Ca2+水平，检测甘胆酸和胆汁酸。
■InsP3RmRNA和蛋白在ICP模型组明显低于正常组,通过qPCR和免疫组织化学确定，分别。血清酶联免疫吸附试验结果显示ICP模型组的甘胆酸和胆汁酸水平明显高于正常组,而Ca2+水平明显降低。Ca2+水平与甘氨胆酸水平呈显著负相关。观察到的Ca2+的减少与总胆汁酸的增加有关,但没有显著的相关性。
■我们的结果表明，ICP模型大鼠肝组织中InsP3R的表达和血清Ca2水平显着降低。此外,发现Ca2水平与甘胆酸水平呈负相关。
这项研究调查了血清Ca2+水平之间的关系，肌醇1,4,5-三磷酸受体(InsP3R)表达与妊娠肝内胆汁淤积症(ICP)的关系.结果表明，与对照组相比，疾病组InsP3R表达和Ca2+显著下降，伴随着甘胆酸和胆汁酸水平升高。Ca2水平与甘胆酸水平呈负相关。这些结果表明，InsP3R表达和Ca2水平的降低可能与ICP的发病有关。该研究为这种疾病的治疗提供了进一步的见解。
