Abstract：
Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in genotoxic response were increased, while in former smokers exposed, proteins (LYZ, ATP1A1, SERPINB3) associated with tissue damage/regeneration, apoptosis inhibition and inflammation that may lead to asthma, COPD or cancer, were upregulated. The identified proteins are potential response and susceptibility/risk biomarkers for SHS exposure.
摘要：
暴露于二手烟（SHS）的非吸烟者存在发展烟草烟雾相关病理的风险。为了研究可用于健康风险评估的SHS暴露的气道分子反应，比较鸟枪蛋白质组学在一组健康的餐厅工作人员的鼻上皮进行，非吸烟者(从未和以前)在工作场所暴露于和未暴露于SHS。HIF1α-糖酵解靶标(GAPDH，TPI)和与异源生物代谢相关的蛋白质，导致癌症的细胞增殖和分化（ADH1C，TUBB4B,EEF2）在非吸烟者中显示出显着的调制。在从未暴露的吸烟者中，谷胱甘肽代谢途径的富集和EEF2调节蛋白质合成的基因毒性反应均增加,而在暴露的前吸烟者中，蛋白质(LYZ，ATP1A1，SERPINB3）与组织损伤/再生相关，凋亡抑制和炎症可能导致哮喘，COPD或癌症，被上调了。鉴定的蛋白质是SHS暴露的潜在反应和易感性/风险生物标志物。
