Abstract：
Malassezia yeasts belong to the normal skin microbiota of a wide range of warm-blooded animals. However, their significance in cattle is still poorly understood. In the present study, the mycobiota of the external ear canal of 20 healthy dairy Holstein cows was assessed by cytology, culture, PCR, and next-generation sequencing. The presence of Malassezia was detected in 15 cows by cytology and PCR. The metagenomic analysis revealed that Ascomycota was the predominant phylum but M. pachydermatis the main species. The Malassezia phylotype 131 was detected in low abundance. Nor M. nana nor M. equina were detected in the samples.
The mycobiota of the external ear canal of healthy cows was assessed by cytology, culture, PCR, and NGS. The presence of Malassezia was detected by cytology and PCR. Ascomycota was the main phylum and M. pachydermatis the main species. The Malassezia phylotype 131 was also detected in the samples.
The mycobiota of the external ear canal of healthy cows was assessed by cytology, culture, PCR, and NGS. The presence of Malassezia was detected by cytology and PCR. Ascomycota was the main phylum and M. pachydermatis the main species. The Malassezia phylotype 131 was also detected in the samples.
摘要：
马拉色菌酵母属于多种温血动物的正常皮肤微生物群。然而,它们在牛中的意义仍然知之甚少。在本研究中,通过细胞学评估了20头健康奶牛的外耳道的分枝杆菌群，文化,PCR,和下一代测序。通过细胞学和PCR在15头牛中检测到马拉色菌的存在。宏基因组分析表明，子囊菌是主要的门，而厚皮杆菌是主要的物种。检测到马拉色菌属型131的丰度较低。在样品中没有检测到M.nana和马匹。
通过细胞学评估了健康奶牛外耳道的分枝杆菌群，文化,PCR,和NGS。通过细胞学和PCR检测马拉色菌的存在。子囊菌是主要门，厚皮杆菌是主要物种。在样品中还检测到马拉色菌属型131。
通过细胞学评估了健康奶牛外耳道的分枝杆菌群，文化,PCR,和NGS。通过细胞学和PCR检测马拉色菌的存在。子囊菌是主要门，厚皮杆菌是主要物种。在样品中还检测到马拉色菌属型131。
