PDF(Pubmed)
Abstract：
UNASSIGNED: Poultry is one of the most prominent sources of Campylobacter jejuni, which is also a major means of transmission to people. Campylobacter jejuni contamination in chicken meat comes from chicken feces because it naturally exists in the intestines of chickens.
UNASSIGNED: The purpose of this study is to identify the antibiotic resistance patterns and genes of C. jejuni, which was found in chickens in Pasuruan, Indonesia.
UNASSIGNED: The samples used in this study were 200 contents of the small intestine of broiler chickens from 40 farms in Pasuruan Regency. The enriched sample was streaked on the selective media of modified charcoal cefoperazone deoxycholate agar containing the CCDA selective supplement. Antimicrobial susceptibility test utilizing the Kirby-Bauer diffusion test method in accordance with Clinical and Laboratory Standards Institute standards. The polymerase chain reaction (PCR) method was used to detect the (hipO), which encodes the C. jejuni strain, fluoroquinolone resistance (gyrA), beta-lactam resistance (blaOXA-61), and tetracycline resistance (tetO) genes.
UNASSIGNED: The findings revealed a 14% (28/200) prevalence of C. jejuni in the small intestine of broiler chickens. These isolates showed high resistance to enrofloxacin (92.9%). All isolates (100%) were susceptible to amoxicillin-clavulanate. The PCR results showed all C. jejuni isolates (100%) detected the gyrA gene, 96.4% detected the blaOXA-61 gene, and 50% detected the tetO gene.
UNASSIGNED: The findings of antimicrobial resistance at a high level from the small intestine of broiler chickens illustrate the potential threat to human health. To lessen the effects now and in the future, coordinated and suitable action is needed, as well as steps to guarantee the poultry industry\'s economic survival and public health insurance.
UNASSIGNED: The purpose of this study is to identify the antibiotic resistance patterns and genes of C. jejuni, which was found in chickens in Pasuruan, Indonesia.
UNASSIGNED: The samples used in this study were 200 contents of the small intestine of broiler chickens from 40 farms in Pasuruan Regency. The enriched sample was streaked on the selective media of modified charcoal cefoperazone deoxycholate agar containing the CCDA selective supplement. Antimicrobial susceptibility test utilizing the Kirby-Bauer diffusion test method in accordance with Clinical and Laboratory Standards Institute standards. The polymerase chain reaction (PCR) method was used to detect the (hipO), which encodes the C. jejuni strain, fluoroquinolone resistance (gyrA), beta-lactam resistance (blaOXA-61), and tetracycline resistance (tetO) genes.
UNASSIGNED: The findings revealed a 14% (28/200) prevalence of C. jejuni in the small intestine of broiler chickens. These isolates showed high resistance to enrofloxacin (92.9%). All isolates (100%) were susceptible to amoxicillin-clavulanate. The PCR results showed all C. jejuni isolates (100%) detected the gyrA gene, 96.4% detected the blaOXA-61 gene, and 50% detected the tetO gene.
UNASSIGNED: The findings of antimicrobial resistance at a high level from the small intestine of broiler chickens illustrate the potential threat to human health. To lessen the effects now and in the future, coordinated and suitable action is needed, as well as steps to guarantee the poultry industry\'s economic survival and public health insurance.
摘要：
■家禽是空肠弯曲菌的最主要来源之一，这也是传播给人们的主要手段。鸡肉中的空肠弯曲杆菌污染来自鸡粪便，因为它天然存在于鸡的肠道中。
■本研究的目的是鉴定空肠弯曲菌的抗生素抗性模式和基因，在Pasuruan的鸡中发现，印度尼西亚。
■本研究中使用的样品是来自PasuruanRegency的40个农场的肉鸡小肠的200内容物。将富集的样品在含有CCDA选择性补充物的改良木炭头孢哌酮脱氧胆酸盐琼脂的选择性培养基上划线。根据临床和实验室标准研究所标准，利用Kirby-Bauer扩散测试方法进行抗菌素敏感性测试。聚合酶链反应(PCR)方法用于检测(hipO),编码空肠弯曲杆菌菌株，氟喹诺酮耐药（gyrA），β-内酰胺抗性（blaOXA-61），和四环素抗性(tetO)基因。
■研究结果表明，肉鸡小肠中空肠弯曲杆菌的患病率为14％（28/200）。这些分离株对恩诺沙星具有较高的耐药性（92.9％）。所有分离株（100％）对阿莫西林-克拉维酸敏感。PCR结果显示所有空肠弯曲菌(100%)均检测到gyrA基因,96.4%检测到blaOXA-61基因,50%检测到tetO基因。
■来自肉鸡小肠的高抗菌耐药性的发现说明了对人类健康的潜在威胁。为了减轻现在和将来的影响，需要采取协调和适当的行动，以及保证家禽业经济生存和公共健康保险的步骤。
■本研究的目的是鉴定空肠弯曲菌的抗生素抗性模式和基因，在Pasuruan的鸡中发现，印度尼西亚。
■本研究中使用的样品是来自PasuruanRegency的40个农场的肉鸡小肠的200内容物。将富集的样品在含有CCDA选择性补充物的改良木炭头孢哌酮脱氧胆酸盐琼脂的选择性培养基上划线。根据临床和实验室标准研究所标准，利用Kirby-Bauer扩散测试方法进行抗菌素敏感性测试。聚合酶链反应(PCR)方法用于检测(hipO),编码空肠弯曲杆菌菌株，氟喹诺酮耐药（gyrA），β-内酰胺抗性（blaOXA-61），和四环素抗性(tetO)基因。
■研究结果表明，肉鸡小肠中空肠弯曲杆菌的患病率为14％（28/200）。这些分离株对恩诺沙星具有较高的耐药性（92.9％）。所有分离株（100％）对阿莫西林-克拉维酸敏感。PCR结果显示所有空肠弯曲菌(100%)均检测到gyrA基因,96.4%检测到blaOXA-61基因,50%检测到tetO基因。
■来自肉鸡小肠的高抗菌耐药性的发现说明了对人类健康的潜在威胁。为了减轻现在和将来的影响，需要采取协调和适当的行动，以及保证家禽业经济生存和公共健康保险的步骤。
