PDF(Pubmed)
Abstract：
UNASSIGNED: Over 75% of clinical microbiological infections are caused by bacterial biofilms that grow on wounds or implantable medical devices. This work describes the development of a new poly(diallyldimethylammonium chloride) (PDADMAC)/alginate-coated gold nanorod (GNR/Alg/PDADMAC) that effectively disintegrates the biofilms of Staphylococcus aureus (S. aureus), a prominent pathogen responsible for hospital-acquired infections.
UNASSIGNED: GNR was synthesised via seed-mediated growth method, and the resulting nanoparticles were coated first with Alg and then PDADMAC. FTIR, zeta potential, transmission electron microscopy, and UV-Vis spectrophotometry analysis were performed to characterise the nanoparticles. The efficacy and speed of the non-coated GNR and GNR/Alg/PDADMAC in disintegrating S. aureus-preformed biofilms, as well as their in vitro biocompatibility (L929 murine fibroblast) were then studied.
UNASSIGNED: The synthesised GNR/Alg/PDADMAC (mean length: 55.71 ± 1.15 nm, mean width: 23.70 ± 1.13 nm, aspect ratio: 2.35) was biocompatible and potent in eradicating preformed biofilms of methicillin-resistant (MRSA) and methicillin-susceptible S. aureus (MSSA) when compared to triclosan, an antiseptic used for disinfecting S. aureus colonisation on abiotic surfaces in the hospital. The minimum biofilm eradication concentrations of GNR/Alg/PDADMAC (MBEC50 for MRSA biofilm = 0.029 nM; MBEC50 for MSSA biofilm = 0.032 nM) were significantly lower than those of triclosan (MBEC50 for MRSA biofilm = 10,784 nM; MBEC50 for MRSA biofilm 5967 nM). Moreover, GNR/Alg/PDADMAC was effective in eradicating 50% of MRSA and MSSA biofilms within 17 min when used at a low concentration (0.15 nM), similar to triclosan at a much higher concentration (50 µM). Disintegration of MRSA and MSSA biofilms was confirmed by field emission scanning electron microscopy and confocal laser scanning microscopy.
UNASSIGNED: These findings support the potential application of GNR/Alg/PDADMAC as an alternative agent to conventional antiseptics and antibiotics for the eradication of medically important MRSA and MSSA biofilms.
UNASSIGNED: GNR was synthesised via seed-mediated growth method, and the resulting nanoparticles were coated first with Alg and then PDADMAC. FTIR, zeta potential, transmission electron microscopy, and UV-Vis spectrophotometry analysis were performed to characterise the nanoparticles. The efficacy and speed of the non-coated GNR and GNR/Alg/PDADMAC in disintegrating S. aureus-preformed biofilms, as well as their in vitro biocompatibility (L929 murine fibroblast) were then studied.
UNASSIGNED: The synthesised GNR/Alg/PDADMAC (mean length: 55.71 ± 1.15 nm, mean width: 23.70 ± 1.13 nm, aspect ratio: 2.35) was biocompatible and potent in eradicating preformed biofilms of methicillin-resistant (MRSA) and methicillin-susceptible S. aureus (MSSA) when compared to triclosan, an antiseptic used for disinfecting S. aureus colonisation on abiotic surfaces in the hospital. The minimum biofilm eradication concentrations of GNR/Alg/PDADMAC (MBEC50 for MRSA biofilm = 0.029 nM; MBEC50 for MSSA biofilm = 0.032 nM) were significantly lower than those of triclosan (MBEC50 for MRSA biofilm = 10,784 nM; MBEC50 for MRSA biofilm 5967 nM). Moreover, GNR/Alg/PDADMAC was effective in eradicating 50% of MRSA and MSSA biofilms within 17 min when used at a low concentration (0.15 nM), similar to triclosan at a much higher concentration (50 µM). Disintegration of MRSA and MSSA biofilms was confirmed by field emission scanning electron microscopy and confocal laser scanning microscopy.
UNASSIGNED: These findings support the potential application of GNR/Alg/PDADMAC as an alternative agent to conventional antiseptics and antibiotics for the eradication of medically important MRSA and MSSA biofilms.
摘要：
■超过75％的临床微生物感染是由伤口或可植入医疗设备上生长的细菌生物膜引起的。这项工作描述了一种新的聚（二烯丙基二甲基氯化铵）（PDADMAC）/藻酸盐涂层的金纳米棒（GNR/Alg/PDADMAC）的开发，该纳米棒可以有效地分解金黄色葡萄球菌的生物膜（S.金黄色葡萄球菌)，引起医院获得性感染的主要病原体。
■GNR是通过种子介导的生长方法合成的，首先用Alg然后用PDADMAC涂覆所得纳米颗粒。FTIR,zeta电位,透射电子显微镜,和紫外-可见分光光度分析进行表征纳米粒子。非包衣GNR和GNR/Alg/PDADMAC在金黄色葡萄球菌预制生物膜中的功效和速度，然后研究了它们的体外生物相容性（L929鼠成纤维细胞）。
■合成的GNR/Alg/PDADMAC（平均长度：55.71±1.15nm，平均宽度：23.70±1.13nm，纵横比：2.35）与三氯生相比，在根除甲氧西林耐药（MRSA）和甲氧西林敏感的金黄色葡萄球菌（MSSA）的预制生物膜方面具有生物相容性和效力，一种用于消毒医院非生物表面上的金黄色葡萄球菌定植的防腐剂。GNR/Alg/PDADMAC的最小生物膜根除浓度(MRSA生物膜的MBEC50=0.029nM;MSSA生物膜的MBEC50=0.032nM)显著低于三氯生(MRSA生物膜的MBEC50=10,784nM;MRSA生物膜的MBEC50=5967nM)。此外，GNR/Alg/PDADMAC在低浓度（0.15nM）下使用时，可在17分钟内有效根除50％的MRSA和MSSA生物膜，与三氯生相似，浓度高得多（50µM）。通过场发射扫描电子显微镜和共聚焦激光扫描显微镜证实了MRSA和MSSA生物膜的崩解。
■这些发现支持GNR/Alg/PDADMAC作为常规防腐剂和抗生素的替代药物用于根除医学上重要的MRSA和MSSA生物膜的潜在应用。
■GNR是通过种子介导的生长方法合成的，首先用Alg然后用PDADMAC涂覆所得纳米颗粒。FTIR,zeta电位,透射电子显微镜,和紫外-可见分光光度分析进行表征纳米粒子。非包衣GNR和GNR/Alg/PDADMAC在金黄色葡萄球菌预制生物膜中的功效和速度，然后研究了它们的体外生物相容性（L929鼠成纤维细胞）。
■合成的GNR/Alg/PDADMAC（平均长度：55.71±1.15nm，平均宽度：23.70±1.13nm，纵横比：2.35）与三氯生相比，在根除甲氧西林耐药（MRSA）和甲氧西林敏感的金黄色葡萄球菌（MSSA）的预制生物膜方面具有生物相容性和效力，一种用于消毒医院非生物表面上的金黄色葡萄球菌定植的防腐剂。GNR/Alg/PDADMAC的最小生物膜根除浓度(MRSA生物膜的MBEC50=0.029nM;MSSA生物膜的MBEC50=0.032nM)显著低于三氯生(MRSA生物膜的MBEC50=10,784nM;MRSA生物膜的MBEC50=5967nM)。此外，GNR/Alg/PDADMAC在低浓度（0.15nM）下使用时，可在17分钟内有效根除50％的MRSA和MSSA生物膜，与三氯生相似，浓度高得多（50µM）。通过场发射扫描电子显微镜和共聚焦激光扫描显微镜证实了MRSA和MSSA生物膜的崩解。
■这些发现支持GNR/Alg/PDADMAC作为常规防腐剂和抗生素的替代药物用于根除医学上重要的MRSA和MSSA生物膜的潜在应用。
