UNASSIGNED: Here, we developed a simple, fast and high-content method named visual Adhesion/Invasion Inhibition Assay (vAIA) to measure the ability of anti-Shigellaantibodies to inhibit bacterial adhesion to and invasion of epithelial cells by using the confocal microscope Opera Phenix.
UNASSIGNED: We showed that vAIA performed well with a pooled human serum from subjects challenged with S. sonnei and that a specific anti-IpaD monoclonal antibody effectively reduced bacterial virulence in a dose-dependent manner.
UNASSIGNED: vAIA can therefore inform on the functionality of polyclonal and monoclonal responses thereby supporting the discovery of pathogenicity mechanisms and the development of candidate vaccines and immunotherapies. Lastly, this assay is very versatile and may be easily applied to other Shigella species or serotypes and to different pathogens.
■这里,我们开发了一个简单的,一种快速且高含量的方法,称为视觉粘附/侵袭抑制测定(vAIA),通过使用共聚焦显微镜OperaPhenix来测量抗志贺拉抗体抑制细菌粘附和侵袭上皮细胞的能力。
我们显示vAIA与来自用S.sonnei攻击的受试者的合并人血清表现良好,并且特异性抗IpaD单克隆抗体以剂量依赖性方式有效降低细菌毒力。
■vAIA因此可以告知多克隆和单克隆反应的功能,从而支持致病性机制的发现以及候选疫苗和免疫疗法的开发。最后,该测定是非常通用的,并且可以容易地应用于其他志贺氏菌属物种或血清型以及不同的病原体。