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Abstract：
OBJECTIVE: Aqueous extract of unripe Musa paradisiaca fruit is commonly used for the treatment of ulcers in eastern Nigeria. This study aimed to assess the acute and subacute effects of an aqueous extract of unripe fruit on male and female fertility in rats.
METHODS: Aqueous extracts obtained by maceration were analyzed for acute and subacute toxicity and for the presence of phytochemical constituents using standard procedures. The extract (100, 500, and 1000 mg/kg) was administered daily to rats of both sexes for 28 d. Blood samples collected on days 0 and 28 were assessed for follicle-stimulating hormone (FSH), luteinizing hormone (LH), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA). Testes and ovaries were harvested for histopathological analysis. Sperm were also collected to determine the sperm count and motility.
RESULTS: Phytochemical screening revealed the presence of saponins, tannins, alkaloids, and resins. After an oral dose of up to 5000 mg/kg, there were no deaths in the acute toxicity test. The extract (500 mg/kg) significantly (P < .05) enhanced sperm count and motility relative to the untreated control; significantly (P < .05) reduced SOD, CAT, and glutathione levels, while significantly (P < .05) elevated LH, FSH, and MDA levels in male and female rats. Histological examination revealed significant structural damage to the ovaries.
CONCLUSIONS: Unripe Musa paradisiaca fruit exhibited an adverse toxicological profile following prolonged administration and caused oxidative stress in rodents.
METHODS: Aqueous extracts obtained by maceration were analyzed for acute and subacute toxicity and for the presence of phytochemical constituents using standard procedures. The extract (100, 500, and 1000 mg/kg) was administered daily to rats of both sexes for 28 d. Blood samples collected on days 0 and 28 were assessed for follicle-stimulating hormone (FSH), luteinizing hormone (LH), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA). Testes and ovaries were harvested for histopathological analysis. Sperm were also collected to determine the sperm count and motility.
RESULTS: Phytochemical screening revealed the presence of saponins, tannins, alkaloids, and resins. After an oral dose of up to 5000 mg/kg, there were no deaths in the acute toxicity test. The extract (500 mg/kg) significantly (P < .05) enhanced sperm count and motility relative to the untreated control; significantly (P < .05) reduced SOD, CAT, and glutathione levels, while significantly (P < .05) elevated LH, FSH, and MDA levels in male and female rats. Histological examination revealed significant structural damage to the ovaries.
CONCLUSIONS: Unripe Musa paradisiaca fruit exhibited an adverse toxicological profile following prolonged administration and caused oxidative stress in rodents.
摘要：
目的：在尼日利亚东部，未成熟Musaparadisiaca果实的水提取物通常用于治疗溃疡。这项研究旨在评估未成熟果实的水提物对大鼠雄性和雌性生育能力的急性和亚急性影响。
方法：使用标准程序分析通过浸渍获得的水提物的急性和亚急性毒性以及植物化学成分的存在。将提取物（100、500和1000mg/kg）每天给予男女大鼠28天。在第0天和第28天收集的血液样品进行卵泡刺激素（FSH）评估，黄体生成素(LH),过氧化氢酶(CAT),超氧化物歧化酶（SOD），和丙二醛(MDA)。收获睾丸和卵巢用于组织病理学分析。还收集精子以确定精子数量和运动性。
结果：植物化学筛选显示存在皂苷，单宁,生物碱，和树脂。口服剂量高达5000mg/kg后，急性毒性试验无死亡病例.相对于未处理的对照，提取物（500mg/kg）显着（P<0.05）提高了精子数量和运动能力；显着（P<0.05）降低了SOD，CAT,和谷胱甘肽水平，而显著（P<0.05）升高的LH，FSH,和MDA水平在雄性和雌性大鼠。组织学检查显示卵巢结构明显受损。
结论：未成熟的木香果实在长期给药后表现出不良的毒理学特征，并在啮齿动物中引起氧化应激。
方法：使用标准程序分析通过浸渍获得的水提物的急性和亚急性毒性以及植物化学成分的存在。将提取物（100、500和1000mg/kg）每天给予男女大鼠28天。在第0天和第28天收集的血液样品进行卵泡刺激素（FSH）评估，黄体生成素(LH),过氧化氢酶(CAT),超氧化物歧化酶（SOD），和丙二醛(MDA)。收获睾丸和卵巢用于组织病理学分析。还收集精子以确定精子数量和运动性。
结果：植物化学筛选显示存在皂苷，单宁,生物碱，和树脂。口服剂量高达5000mg/kg后，急性毒性试验无死亡病例.相对于未处理的对照，提取物（500mg/kg）显着（P<0.05）提高了精子数量和运动能力；显着（P<0.05）降低了SOD，CAT,和谷胱甘肽水平，而显著（P<0.05）升高的LH，FSH,和MDA水平在雄性和雌性大鼠。组织学检查显示卵巢结构明显受损。
结论：未成熟的木香果实在长期给药后表现出不良的毒理学特征，并在啮齿动物中引起氧化应激。
