Abstract：
Toll-like receptors (TLRs) are one of the extensively studied pattern recognition receptors (PRRs) and play crucial roles in the immune responses of vertebrates and invertebrates. In this study, 14 TLR genes were identified from the genome-wide data of Octopus sinensis. Protein structural domain analysis showed that most TLR proteins had three main structural domains: extracellular leucine-rich repeats (LRR), transmembrane structural domains, and intracellular Toll/IL-1 receptor domain (TIR). The results of subcellular localization prediction showed that the TLRs of O. sinensis were mainly located on the plasma membrane. The results of quantitative real-time PCR (qPCR) showed that the detected TLR genes were differentially expressed in the hemolymph, white bodies, hepatopancreas, gills, gill heart, intestine, kidney, and salivary gland of O. sinensis. Furthermore, the present study investigated the expression changes of O. sinensis TLR genes in hemolymph, white bodies, gills, and hepatopancreas in different phases (6 h, 12 h, 24 h, 48 h) after stimulation with PGN, poly(I: C) and Vibrio parahaemolyticus. The expression of most of the TLR genes was upregulated at different time points after infection with pathogens or stimulation with PAMPs, a few genes were unchanged or even down-regulated, and many of the TLR genes were much higher after V. parahaemolyticus infection than after PGN and poly(I:C) stimulation. The results of this study contribute to a better understanding of the molecular immune mechanisms of O. sinensis TLRs genes in resistance to pathogen stimulation.
摘要：
Toll样受体(TLRs)是被广泛研究的模式识别受体(PRRs)之一,在脊椎动物和无脊椎动物的免疫应答中起着至关重要的作用。在这项研究中,从章鱼全基因组数据中鉴定出14个TLR基因。蛋白质结构域分析显示,大多数TLR蛋白具有三个主要结构域:胞外富含亮氨酸重复序列(LRR),跨膜结构域，和细胞内Toll/IL-1受体结构域(TIR)。亚细胞定位预测结果表明，中华牛的TLRs主要位于质膜上。实时定量PCR(qPCR)结果显示,检测到的TLR基因在血淋巴组织中差异表达,白色的身体,肝胰腺,ill,刺心,肠,肾,和中华民国的唾液腺。此外，本研究调查了O.sinensisTLR基因在血淋巴中的表达变化，白色的身体,ill,和肝胰腺在不同阶段(6h，12h,24h,48h)用PGN刺激后，聚（I：C）和副溶血性弧菌。大多数TLR基因的表达在病原体感染或PAMPs刺激后的不同时间点上调。一些基因没有改变，甚至下调，副溶血弧菌感染后，许多TLR基因比PGN和poly（I：C）刺激后高得多。这项研究的结果有助于更好地理解O.sinensisTLRs基因对病原体刺激的抗性的分子免疫机制。
