PDF(Pubmed)
Abstract：
We established primary porcine nasal, tracheal, and bronchial epithelial cells that recapitulate the physical and functional properties of the respiratory tract and have the ability to fully differentiate. Trans-well cultures demonstrated increased transepithelial electrical resistance over time the presence of tight junctions as demonstrated by immunohistochemistry. The nasal, tracheal, and bronchial epithelial cells developed cilia, secreted mucus, and expressed sialic acids on surface glycoproteins, the latter which are required for influenza A virus infection. Swine influenza viruses were shown to replicate efficiently in the primary epithelial cell cultures, supporting the use of these culture models to assess swine influenza and other virus infection. Primary porcine nasal, tracheal, and bronchial epithelial cell culture models enable assessment of emerging and novel influenza viruses for pandemic potential as well as mechanistic studies to understand mechanisms of infection, reassortment, and generation of novel virus. As swine are susceptible to infection with multiple viral and bacterial respiratory pathogens, these primary airway cell models may enable study of the cellular response to infection by pathogens associated with Porcine Respiratory Disease Complex.
摘要：
我们建立了初级猪鼻，气管,和概括呼吸道的物理和功能特性并具有完全分化能力的支气管上皮细胞。通过免疫组织化学证明，跨孔培养物显示出随着时间的推移，紧密连接的存在会增加跨上皮电阻。鼻部，气管,支气管上皮细胞发育出纤毛，分泌的粘液，并在表面糖蛋白上表达唾液酸，后者是甲型流感病毒感染所必需的。猪流感病毒被证明在原代上皮细胞培养物中有效复制，支持使用这些培养模型评估猪流感和其他病毒感染。初级猪鼻，气管,和支气管上皮细胞培养模型能够评估新兴和新型流感病毒的大流行潜力以及机制研究，以了解感染机制，重新分类，和新型病毒的产生。由于猪容易感染多种病毒和细菌呼吸道病原体，这些主要气道细胞模型可以研究猪呼吸道疾病复合体相关病原体对感染的细胞反应。
