PDF(Pubmed)
Abstract：
Southern Africa Territories 2 (SAT2) foot-and-mouth disease (FMD) has crossed long-standing regional boundaries in recent years and entered the Middle East. However, the existing vaccines offer poor cross-protection against the circulating strains in the field. Therefore, there is an urgent need for an alternative design approach for vaccines in anticipation of a pandemic of SAT2 Foot-and-mouth disease virus (FMDV). The porcine parvovirus (PPV) VP2 protein can embed exogenous epitopes into the four loops on its surface, assemble into virus-like particles (VLPs), and induce antibodies and cytokines to PPV and the exogenous epitope. In this study, chimeric porcine parvovirus VP2 VLPs (chimeric PPV-SAT2-VLPs) expressing the T-and/or B-cell epitopes of the structural protein VP1 of FMDV SAT2 were produced using the recombinant pFastBac™ Dual vector of baculoviruses in Sf9 and HF cells We used the Bac-to-Bac system to construct the recombinant baculoviruses. The VP2-VLP--SAT2 chimeras displayed chimeric T-cell epitope (amino acids 21-40 of VP1) and/or the B-cell epitope (amino acids 135-174) of SAT FMDV VP1 by substitution of the corresponding regions at the N terminus (amino acids 2-23) and/or loop 2 and/or loop 4 of the PPV VP2 protein, respectively. In mice, the chimeric PPV-SAT2-VLPs induced specific antibodies against PPV and the VP1 protein of SAT2 FMDV. The VP2-VLP-SAT2 chimeras induced specific antibodies to PPV and the VP1 protein specific epitopes of FMDV SAT2. In this study, as a proof-of-concept, successfully generated chimeric PPV-VP2 VLPs expressing epitopes of the structural protein VP1 of FMDV SAT2 that has a potential to prevent FMDV SAT2 and PPV infection in pigs.
摘要：
南部非洲第二领土（SAT2）口蹄疫（FMD）近年来已越过长期的区域边界，进入中东。然而,现有的疫苗对该领域的流行菌株提供较差的交叉保护。因此,在预期SAT2口蹄疫病毒(FMDV)大流行时,迫切需要一种替代的疫苗设计方法.猪细小病毒(PPV)VP2蛋白可以将外源表位嵌入其表面的四个环,组装成病毒样颗粒(VLP)，并诱导针对PPV和外源表位的抗体和细胞因子。在这项研究中,使用Sf9和HF细胞中的杆状病毒的重组pFastBac™双重载体产生表达FMDVSAT2的结构蛋白VP1的T和/或B细胞表位的嵌合猪细小病毒VP2VLP（嵌合PPV-SAT2-VLP）。我们使用Bac-to-Bac系统构建重组杆状病毒。VP2-VLP-SAT2嵌合体展示了SATFMDVVP1的嵌合T细胞表位（VP1的氨基酸21-40）和/或B细胞表位（氨基酸135-174），通过取代PPVVP2蛋白的N末端（氨基酸2-23）和/或环2和/或环4的相应区域，分别。在老鼠身上,嵌合PPV-SAT2-VLP诱导针对PPV和SAT2FMDV的VP1蛋白的特异性抗体。VP2-VLP-SAT2嵌合体诱导针对PPV的特异性抗体和FMDVSAT2的VP1蛋白特异性表位。在这项研究中,作为一个概念证明，成功地产生了表达FMDVSAT2的结构蛋白VP1表位的嵌合PPV-VP2VLP，该表位具有预防猪中FMDVSAT2和PPV感染的潜力。
